To observe the influence of adiponectin with different doses on apolipoprotein A5 (apoA5)mRNA and protein expression levels and triglyceride(TG) content in HepG2 cells and to investigate the relationship between adiponectin  and TG and to establish  foundation for study on the mechanism of adiponectin in decreasing TG content.Methods HepG2 cells were divided into control group，low dose adiponectin group,middle dose adiponectin group and high dose adiponectin  group.The HepG2 cells in various groups were treated with  different doses of adiponectin （0,12.5,25.0 and 50.0 mg·L^-1）for  24 h,then the TG  contents in HepG2 cells in various groups were detected with   enzyme method.The  expression levels of apoA5 mRNA in  HepG2 cells were  detected with  real time  fluorescence quantitative PCR  method.The  expression levels of apoA5 protein in HeG2 cells were  detected with  Western blotting method. Results Compared with control group,the TG contents in HepG2 cells in different doses of adiponectin  groups were decreased obviously (P<0.05）.Compared with low dose group,the TG  contents in HepG2 cells in middle dose group and high dose group were decreased obviously (P<0.05）,but there was no significant difference between  high dose group and middle dose group(P>0.05).Compared with control group,the gene and 　protein expression levels of apoA5 in HepG2 cells in different doses of adiponectin  groups were increased obviously (P<0.05）.Compared with low dose group,the gene and protein expression levels of apoA5 in HepG2 cells in middle dose group and high dose group were increased obviously (P<0.05）,but there was no significant difference between  high dose group and middle dose group(P>0.05).  Conclusion Adiponectin may decrease the TG content in HepG2 cells in an dose-dependent manner.It indicats that the mechanism of adiponectin in decreasing TG content may be related to   increasing the  expression of apoA5 in HepG2 cells.

To measure the changes of the radiosensitivity in human lung adenocarcinoma A549 cells transfected with pEgr1-hsTRAIL plasmid and the effect on death receptor (DR) 4 and DR5 expressions,and to explore the radiosensitizing effect of pEgr1-hsTRAIL plasmid and possible mechanism on inducing apoptosis.Methods There were normal control,pEgr1-hsTRAIL,6 Gy X-rays,and pEgr1-hsTRAIL + 6 Gy X-rays groups in the experiment.After the A549 cells were transfected with liposome,and irradiated with X-rays,colony formation assay was used to measure the radiosensitivity,and reverse transcription PCR (RT-PCR) was performed to detect the  DR4 and DR5 mRNA expressions,and Western blotting was applied to determine the DR4 and DR5 protein expressions.Results The D0 values of  A549 cells in normal control  group and pEgr1-hsTRAIL group were 3.26 and 1.91 Gy,respectively,it indicated that pEgr1-hsTRAIL plasmid could enhance the radiosensitivity in A549 cells.The RT-PCR results showed that as compared with normal control group,the DR4 and DR5 mRNA expression levels  in pEgr1-hsTRAIL group had no significant change,but those in 6 Gy X-rays group were increased significantly (P<0.05),and those in pEgr1-hsTRAIL + 6 Gy X-rays group were also increased significantly (P<0.05);the DR5 mRNA expression level in pEgr1-hsTRAIL + 6 Gy X-rays  group was higher than that in 6 Gy X-rays group (P<0.05).The Western blotting results showed that the DR4 and DR5 protein expressions in pEgr1-hsTRAIL group did not change obviously  compared with  normal control group,but those in 6 Gy X-rays and pEgr1-hsTRAIL + 6 Gy X-rays  groups were increased,and the DR5 protein expression in pEgr1-hsTRAIL +6 Gy X-rays group was increased mostly.Conclusion The recombinant plasmid pEgr1-hsTRAIL can enhance the radiosensitivity of A549 cells,and has the enhancing effect on DR5 expression induced by radiation,but no same effect on DR4 expression.

To construct the CHO expression system of interferon-β1a (IFN-β1a) gene and to explore the expression efficacy of human IFN-β1a gene in eukaryotic cells. Methods The synthesized IFN-β1a gene was amplified and cloned into plasmid pSV2-dhfr.Cysteine 17 was mutated to serine.The constructed recombinant plasmid pSV2-dhfr-IFN-β1a was transfected to CHO-dhfr－ cells,and the monoclonal cell strains growing stably were obtained by MTX pressure screening.The genomic DNA of transfected cells was extracted;target gene was identified by PCR.The antiviral activity of IFN-β1a in transfected cells was determined by Wish cytopathic inhibition test.Results  Both PCR and restriction enzyme analysis showed that the recombinant plasmid pSV2-dhfr-IFN-β1a was constructed correctly.The IFN-β1a gene was amplified by using the genomic DNA of transfected cells as template.The expressed IFN-β1a showed high antiviral activity up to 3×105 IU·mL^-1.Conclusion A eukaryotic expression vector of IFN-β1a gene is successfully constructed,and the IFN-β1a protein with biological activity is expressed in CHO cells.

To explore the effect on obesity by deep brain stimulation(DBS) in the rat nucleus accumbens (NAc) shell,and to elucidate the feasibility of DBS for obesity treatment.
Methods 30 rats were randomly divided into DBS group(n=15) and control group(n=15).High fat feed was given from the beginning,and electrode fixing device and electrode were implanted stereotacticly.The rats in DBS group were given high frequency stimulation for 7 d,the rats in control group were not given stimulation.After electrode pulled-out,the rats in two groups were observed for another 28 d.In the whole experimental course (57 d),the body weight,food intake,and water intake were monitored every day.A comprehensive comparison of weight gain rate,food intake and water intake was conducted.Results  From the fifth day after NAc shell stimulation,the weight gain rates of  the rats in DBS group were lower than those in control group (P<0.05).At the end of the experiment,the weight gain rate reached to 10.2% and 35.7% in DBS group and control group.In the stimulation period,a food intake reduction was observed in DBS group（P<0.05）.After  stimulation,the difference of food intake was no longer existed.The water intake during the whole experiment had no obvious changes in both groups(P>0.05).Conclusion NAc shell could be an ideal target for DBS in treatment of obesity in rats.On the one hand,it can limit feeding behavior,and reduce food intake;on the other hand,it may interact with energy homeostasis system,increase energy expenditure and decrease weight growth of rats.

To explore the effect of cadmium telluride quantum dots (CdTe QDs) on the proliferation and osteogenesis differentiation of rat bone marrow mesenchymal stem cells (BMSCs),and to illustrate the toxicity of CdTe QDs in vitro,and to provide experimental evidence for application of CdTe QDs for living cell labeling marker.Methods Photoluminescence detector was used to detect photoluminescence emission spectra of the CdTe QDs in order to determine the dispersion of CdTe QDs in DMEM/F-12 medium.The rat BMSCs were cultured,and MTT assay was performed to examine the effects of different concerntrations (0,0.195 3,0.390 6,0.781 3,1.562 5,3.125 0,6.250 0,12.500 0,25.000 0,and 50.000 0 nmol·L^-1) of CdTe QDs on the proliferation of BMSCs;in vitro hexadecadrol,sodium glycerophosphate and vitamin C were used to induce osteogenesis differentiation. Alizarin red staining was used to display calcium nodus.The concentration inducing 50% of inhibition of proliferation (IP50) and the concentration inducing 50% of inhibition of differentiation (ID50) were calculated.Results Photoluminescence emission spectra showed that CdTe QDs were well dispersed in DMED-F/12 medium without segregation.The longer the exposure time went,the less the cell viability remained.The regression coefficients of 24,48 and 72　h exposure were separately －23.96,－29.61 and －24.30  (P<0.05);and the IP50 of each time was separately 7.25,1.63， and 0.67 nmol·L^-1.And the higher the exposure dose was,the lower the osteogenesis differentiation rate was.The regression coefficient of 48　h exposure was －56.15 (P<0.05),and the ID50 was 0.041 2 nmol·L^-1,and the ratio of IP50/ ID50 was 39.56.Conclusion At a certain concentration range (0.012 2－50.000　0 nmol·L^-1),CdTe QDs could inhibit the proliferation and osteogenesis differentiation of BMSCs.When CdTe QDs are used as living cell labeling marker,their influence in cell proliferation and differentiation should be considered.

To investigate the inhibitory effect of indomethacin combined with radiation on proliferation of HL-60 cells and to provide  basis for study on antitumor therapy.
Methods The HL-60 cells were exposed to indometnacin at 0,20,40,60,80,and 100  μmol·L^-1 together with 3 Gy X-rays radiation and cultivated for 24 h.MTT and Trypan blue exclusion experiments were used to detect the inhibitory rate of cell proliferation and viability respectively.Real-time PCR was used to detect the changes of cell proliferation and apoptosis-related gene PCNA and Caspase-3 mRNA expressions.Results  Compared with control group,the inhibitory rates of proliferation of HL-60 cells in different doses of indomethacin groups were increased significantly（P<0.01）,especially in 80  μmol·L^-1 indomethacin group.Compared with control,indomethacin (80  μmol·L^-1) and 3 Gy X-rays radiation groups,the inhibitory rate of cell viability of HL-60 cells in 80  μmol·L^-1 indomethacin combined with radiation group was  increased significantly（P<0.01）.Compared with control,indomethacin (80 μmol·L^-1) and 3 Gy X-rays radiation groups,the expression of PCNA mRNA in 80 μmol·L^-1 indomethacin combined with radiation group  was decreased and the expression of Caspase-3 mRNA was increased significantly （P<0.01）.Conclusion Indomethacin can enhance the inhibitory effect of radiation on roliferatio
n of HL-60 cells.

To study the cytotoxicity and effect on gap junction intracellular communication(GJIC) of SiO2 nanoparticles in HL-7702 cells,and to provide experimental basis for toxicity assessment and the security applications of SiO2 nanoparticles. Methods Transmission electron microscope(TEM) was used to characterize two kinds of SiO2 nanoparticles,verifying their size,dispersion and shape;dynamic light scattering (DLS) method was used to analyze the water dispersion and culture medium dispersion　of the SiO2 nanoparticles;MTT assay was carried out to examine the cytotoxicities of the two sizes SiO2 nanoparticles on the cells;lactate dehydrogenase(LDH) release assay was performed to examine the integrity nano of the cell membrane;Scrape-loading and dye transfer assay was performed to examine the effect of SiO2 nanoparticles on GJIC.Results Based on the result of TEM,two kinds of SiO2 nanoparticles were spherically shaped,uniformly sized and sporadically dispersed;the statistical analysis results showed the diameters of the two nanoparticles were (447.60±20.78) nm and (67.42±5.69) nm,respectively,thus they could be categorized as submicron scale and nano scale.The DLS  method results manifested that the hydration nanoparticle sizes of the two SiO2 nanoparticles were（684.37±18.76） nm，（128.31±7.64） nm in high purity water and （697.02±19.57） nm，（133.74±8.97） nm in RPMI-1640 solution,all the two nanoparticles were well dispersed without aggregation.MTT assay indicated that 24 h after  treatment of SiO2 nanoparticles,the cell viabilities were affected by both the size and the dose of the SiO2 nanoparticles;the higher the dose was,the less viability the cells exhibited.Moreover,the nano scale particles inflicted more damage to the cells.LDH release assay indicated that the SiO2 particles could also damage the cell membrane in a dose-dependent and size-dependent way.Scrape-loading and dye transfer assay indicated that the nano scale particles could cause GJIC inhibition in a dose-dependent way;and when at the same dose,the nanoparticles could cause a more obvious inhibition of GJIC than the submicron particles.Conclusion SiO2 nanoparticles have cytotoxicity on HL-7702 cells,and would cause GJIC inhibition.

To research the cloning and expression of the megakaryocyte protein tyrosine phosphatase 2(PTPMEG2) and its regulatory role in osteoporosis development.Methods
The purified PTPMEG2 was used to prepare PTPMEG2 polyclonal antibody.30 female SD rats were rardomly divded into model group(n=15) and control group(n=15).The retinoic acid gavage method was used to construct rat osteoporosis models.The rats in control group were gavaged with saline.The acid phosphatase (ACP) and alkaline phosphatase (ALP) levels in rat serum in two groups were detected.The expression levels of PTPMEG2 in some rat tissues were detected by Western blotting method.The expression level of PTPMEG2 in bone marrow cells (BMCs),red blood cells and bone marrow stromal cells (BMSCs)were detected and compared.Results The PTPMEG2 polyclonal antibody was successfully prepared.The levels of serum ACP and ALP of rats in model group were higher than those in control group (P<0.01).The expression level of PTPMEG2 in kidney tissue of rats in model group was higher than that in control group(P<0.05),but the expression level in muscle tissue of rats in model group was lower than that in control group(P<0.05).Compared with control group,the expression levels of PTPMEG2 in red blood cells and BMCs of rats in model group has no significant changes,but the expression level of PTPMEG2 in rat BMSCs in model group was significantly increased. Conclusion The expression level of PTPMEG2 in BMCs of osteoporosis rats is increased,and mostly in mature bone cells differentiated in bone marrow;and  PTPMEG2 has important role in osteoporosis development.

To investigate the influence of all-trans retinoic acid (ATRA) in proliferation,migration and invasion of human hepatocellular carcinoma SMMC-7721 cells and to clarity its molecular mechanism.Methods The SMMC-7721 cells in logarithmic phase were divided into blank control group and 10,20,and 40 μmol?L-1 ATRA groups.The  proliferation ability of SMMC-7721 cells was   examined by MTS／PMS assay,and the migration and invasion abilities of SMMC-7721 cells were detected by  Wound Healing assay and Transwell assay，and the expressions of  MMP-9  mRNA and protein in SMMC-7721 cells were determined by Real-time PCR and Western blotting methods.Results Compared with blank control group,the proliferation rates in 20 and 40 μmol?L-1 ATRA groups were decreased significantly（P<0.01）.Wound Healing assay discovered that the scuffing distance of SMMC-7721 cells exposed to 20 and 40 μmol?L-1 ATRA for 24 h was significantly increased compared with blank control group（P<0.05）.Transwell assay showed that the numbers of SMMC-7721 cells migrated through the matrigel in 10,20,and 40 μmol?L-1 ATRA groups  24 h after treatment were obviously decreased compared with blank control group (P<0.05)．The Real-time PCR and Western blotting results showed that the expressions of MMP-9 mRNA and protein in SMMC-7721 cells in  20 and 40 μmol?L-1 ATRA groups 24 h after treatment were significantly decreased compared with blank control group (P<0.01)．Conclusion ATRA may inhibit the proliferation,migration and invasion of  human hepatocellular carcinoma SMMC-7721 cells,which may be related to the down-regulation of MMP-9.

To study the role of CC chemokine ligand-20(CCL20)and dendritic cells(DCs) in the pathogenesis of chronic obstructive pulmonary disease (COPD) and the influence of anti-rat CCL20 antibody treatment on the expressions of surface factors of DCs in peripheral blood and brochoalveolar lavage fluid(BALF). Methods  48 Wistar rats were randomly divided into control group(n=16)，COPD model group(n=16) and CCL20 inhibited model group(n=16).The rat COPD models were established via cigarette inhalation about for  4 weeks and intratracheal administration of lipopolysaccharide (LPS) solution twice totally.The patholgical changes of rat lung tissues in three groups were observed with HE staining.The CCL20 contents in peripheral blood and BALF of rats in three groups were detected with ELISA.The percentages of CD80,CD86,OX-62 and MHC-Ⅱ of DCs were analyzed by FCM.Results  The HE staining results suggested that there were airway inflammation and development of emphysema of rats in COPD group and CCL20 inhibited model  group,but they  were reduced in latter group.compared with control group,the contents of CCL20 in BALF and peripheral blood in COPD group and CCL20　inhibited model group were significantly increased（P<0.05）;compared with COPD group,the content of CCL20 in BALF of rats in CCL20 inhibited model group was decreased（P<0.05）,but the content of CCL20 in peripheral blood had no change.Compared with control group,the percentages of CD80,CD86 and OX-62 in peripheral blood of rats in COPD group were increased（P<0.05);compared  with COPD group, the percentages of CD80 and OX-62 of rats in CCL20 inhibited model group were decreased（P<0.05）.Compared with control group,the percentages of CD80 and CD86 in BALF of rats in COPD group and CCL20 inhibited model group were increased（P<0.05）;compared  with COPD group, the percentage of CD80 in CCL20 inhibited model group was decreased（P<0.05）.Conclusion CCL20 may be involved in the pathogenesis of COPD,anti-rat CCL20 antibody may play a inhibitory effect in this pathogenesis,it can decrease the number of DCs in the peripheral blood and  the airway of COPD,and the downgulation of DCs may  help to reduce the systemic inflammatory response of COPD.

To establish  rat models of  chronic psychological stress and to study the effects of Jiaweisinisan(JWSNS) on gastric emptying rate and small intestinal propulsion ratio,cyclic nucleotide system in rat hypothalamus ( cAMP,CGMP )，and mRNA expressions of gastrin receptor (GASR) in stomach tissue and vasoactive intestinal peptide II receptor ( VIPR2) in jejunal tissue of rat models,and to elucidate the mechanism of JWSNS in effecting stress-induced gastrointestinal dysfunction. Methods 60 rats were randomly divided into normal group,model group,small,middle,big doses of JWSNS  groups,and Xishabilly group,10 in each group.The rat models were set up by chronic psychological stress method,after JWSNS intervention,the gastrointestinal motility was detected,and radioimmunoassay was used to detect the changes of cAMP and cGMPcontents in hypothalamus of rats，and the mRNA expression changes of GASR in stomach tissue and VIPR2 in jejunal tissue of rats were measured with RT-PCR method.
Results  Compared with model group,the contents of hypothalamus cGMP and cAMP in treatment groups were decreased,there were  significant differences (P<0.05or P<0.01);compared with Xishabilly group,the contents of hypothalamus cGMP and cAMP in big dose of JWSNS  group were decreased,there were significant differences(P<0.05　or　P<0.01).Compared with model group,the gastric emptying rate and small intestinal propulsion ratio in treatment groups were significantly increased (P<0.05　or P<0
.01);compared with Xishabilly group,the gastric emptying rate in  big dose of JWSNS group was not significantly increased (P>0.05),and the small intestinal propulsion ratio was increased significantly (P<0.05). Compared with model group,the GASR mRNA expression levels in stomach tissue in treatment groups were increased,and the VIPR2 mRNA expression levels in jejunal tissue were decreased,the differences were statistically significant (P<0.05　or　P<0.01);the GASR mRNA expression levels in middle   and big doses of JWSNS  groups were higher  than that in Xishabilly group (P<0.05 or　P<0.01),and the VIPR2 mRNA expression level in jejunal tissue in  big dose of JWSNS group was lower than that in  Xishabilly group ( P<0.01).
Conclusion The cyclic nucleotide system (cAMP,CGMP) abnormalities can affect the gastrointestinal function and JWSNS can regulate chronic psychological stress response by influencing cyclic nucleotide system,thereby improve the gastrointestinal function，and improve the expressions of VIPR2 and GASR mRNA in stomach and jejunum tissues.


Abstract:Objective  To explore the effect of  astragalus combined with insulin on insulin resistance in diabetic rats and to clarify the action mechanism.Methods The diabetic rat models induced by STZ were randomly divided into  diabetic model(DM) group,astragalus(RA) group,insulin(Ins) group and astragalus combined with insulin(RA+Ins) group.At the same time the normal rats were used as control group.After treatment for 8  weeks,the levels of fasting blood glucose(FBG) and fasting insulin(FINS) of rats were measured,and the contents of MDA and TNF-α in serum were detected,and the levels of insulin receptor substrate-1(IRS-1) and p38MAPK in skeletal muscle were examined by Western blotting.Results Compared with control group,the levels of FBG of rats in DM，RA，Ins and RA+Ins groups were significantly increased (P<0.05)，and the FBG levels in RA,Ins  and RA+Ins groups  were significantly decreased compared with DM group(P<0.05);the FBG level in  RA+Ins group was significantly decreased compared with RA and Ins groups(P<0.05).Compared with DM,RA and control groups,the levels of FINS in Ins and RA+Ins groups  were increased(P<0.05),and the FINS level in  RA+Ins group was decreased compared with Ins group(P<0.05).Compared with control group,the levels of MDA and TNF-α in DM,RA,Ins and RA+Ins groups were significantly increased(P<0.05),and they were  lower in RA,Ins and RA+Ins groups compared with DM group(P<0.05);compared with RA and Ins groups,the MDA and TNF-α in RA+Ins were decreased(P<0.05).The activities of SOD  in DM, RA,Ins and RA+Ins groups were significantly decreased compared with control group(P<0.05),and they were higher in RA,Ins and RA+Ins groups compared with DM group(P<0.05);compared with Ins and RA groups,it was increased in RA+Ins group(P<0.05).The expression levels of IRS-1 in DM,RA and RA+Ins groups were decreased compared with control group(P<0.05),but it was higher in RA+Ins  than DM,RA and Ins groups(P<0.05).The expression levels of p38MAPK in  DM,RA,Ins and RA+Ins groups were increased compared with control group(P<0.05),but  it was lower in RA+Ins group than DM,RA and Ins groups(P<0.05).Conclusion Astragalus combined with insulin significantly improve   the oxidative stress and insulin resistance in  diabetic rats,and its efficacy is better than insulin alone.

Abstract:Objective  To explore the effect of  Soybean saponins(SS)  on the expressions of  NOX4 and p22phox in the myocardium tissues of diabetic rats,and to clarify its protective effect on myocardium.Methods Male Wistar rat models of diabetes mellitus were randomly divided into diabetes  group and SS treatment group,the rats in two groups were given daily saline and SS(10 mg/Kg)orally for 3 months;the rats treated with 0.1 mol/L citrate buffer were used as control group and administrated with saline for 3 months;then the animals were killed and the  myocardium tissues were obtained.The NOX4 and p22phox mRNA expressions in rat myocardium tissues were detected with real-time fluorescence quantitative polymerase chain reaction (PCR).Immunohistochemistry was used to detect the expressions of copper-zinc superoxide dismutase (Cu-Zn-SOD) and connective tissue growth factor(CTGF) in myocardium tissues.HE staining was performed to observe the pathological changes of myocardium.Results  Compared with control group,the expression levels of NOX4 mRNA,p22phox mRNA and CTGF protein in myocardium tissues of rats in diabetes  group were significantly increased(P<0.01),and the Cu-Zn-SOD protein expression level was significantly decreased(P<0.01).Compared with diabetes  group,the expression levels of NOX4 mRNA,p22phox mRNA and CTGF protein in myocardium tissues of rats in SS treatment group were significantly reduced(P<0.01),but the Cu-Zn-SOD protein expression level was significantly increased(P<0.01);there was no significant difference of the indexes mentioned above between control group and SS treatment group (P>0.05).HE staining showed the rat myocardial cells in  control group were tightly and neatly packed,but the rat myocardial cells in diabetes  group arranged loosely and disorderly with infiltration of inflammatory cells in mesenchyma,and the myocardial cells in SS treatment group were also tightly and neatly packed.Conclusion SS can reduce the expression levels of NOX4 mRNA,p22phox mRNA and CTGF protein  in myocardium tissues of diabetic rats,increase the expression level of Cu-Zn-SOD protein and alleviate myocardial damage induced by  oxidative stress,which has a protective effect on the myocardium.

Abstract:Objective  To explore the effect of Juglone on proliferation of liver cancer HepG2 cells and to clarify its possible mechanism. Methods The human hepatoma HepG2 cells were divided into different doses of Juglone（40，80，120，160 and 200 μmol/L) groups and control group.The proliferation inhibition of Juglone on HepG2 cells was measured by MTT assay,and IC50 was calculated based on the effective concentration.Optical microscope was used to observe the morphological changes of HepG2  cells．Meanwhile the expression of Caspase-3 protein was analyzed by immunocytochemistry.The cell cycle of HepG2 cells was detected by flow cytometry．Results The IC50 of Juglone was 139.888 1 μmol/L.Compared with control group, the inhibitory rates of proliferation of HepG2 cells in different doses of Juglone groups  were increased significantly(P<0.05 or P<0.01) in a  dose-dependent manner.The morphological observation showed that the cells in different does of Juglone groups were smaller and the  connection disappeared.In 120 μmol/L Juglone group the cell cycle of HepG2 cells  was changed and the cells were  markedly blocked in the G2/M phase.The immunocytochemistry staining results indicated that the Caspase-3 protein  expression in 120 μmol/L Juglone group was higher than that in control group.Conclusion  Juglone could induce the apoptosis of HepG2 cells,and the mechanism may be related to Caspase pathway.

Abstract:Objective To explore the effect of allogenic platelet-rich plasma(PRP) extraction solution on the proliferation of adipose-derived stem cells(ADSCs) in vitro and to provide an experimental evidence for wound healing by allogenic PRP extracting solution.Methods The adipose tissue derived from SD rats was isolated,cultured and passaged.The cells harvested above were cultured respectively in adipose-,osteo- and neuro- lineages inducing culture solution.The rats’ blood was extracted and prepared by three times’ centrifugation for making PRP and different concentrations of allogenic PRP extraction solution (6.67%,3.35%,and 1.67% volume fractions) were prepared by DMEM/F12. The third passage of ADSCs were divided into 4 groups.The ADSCs in experimental groups (A,B and C groups) were respectively cultured with 6.67%,3.35% and 1.67% volume fractions of PRP extraction solution.The cells in control group (D group) were cultured with 10% fetal bovine serum and DMEM/F12.The proliferative activities of the ADSCs were detected by MTT after 24 and 48 h culture. Results The harvested cells appeared to be adherent and fibroblast-like,which were successfully obtained and culured from rat adipose tissue.The cells could differentiate into adipocytes,osteocytes and neurocytes by adipose-inducing,osteo-inducing,and neuro-inducing.It was proved that the cells had the potential of differentiating into adipose-,osteo- and neuro- lineage cells and the harvested cells were ADSCs.The A values assayed by MTT after 24 h in  A,B,C and D groups were 0.434 3±0.084 3,0.351 6±0.076 6,0.258 1±0.060 0, and 0.259 2±0.073 6,respectively;and the values after 48 h were 1.018 6±0.443 6,0.741 7±0.109 7,0.367 8±0.034 2,and 0.395 7±0.106 2,respectively.The A values in A group and B group were significantly higher than those in C group and D group (P<0.05),but there was no statistically significant difference between C and D groups(P>0.05).Compared with D group,the proliferation rates in A group and B group were significantly increased,but it showed negative increase in C group.Conclusion Allogenetic PRP extraction solution with suitable concentration can enhance the proliferation of  ADSCs  in vitro
,which is expected to accelerate wound repaire.

Abstract:Objective To observe the therapeutic effect of Simiaoyong’an Decoction (SMYA) on the rat models with thromboangiitis obliterans (TAO),and to explore the action mechanism.Methods The rats were randomly divided into 6 groups:sham operation group,model group,mailuoning group(MLN 2.7 g/kg),high,medium and low doses of SMYA groups (24.3,16.2,8.1 g/kg). The  TAO model was prepared by injecting sodium laurate into the femoral artery of rats.The rats were administered by intragastric administration after prepared model.After 15 d administration,the pathological changes of blood vessel and surrounding tissue were observed with HE staining,the contents of IL-6,TNF-α,CRP,TXB2 and 6-K-PGF1α in plasma were measured by enzyme-linked immunosorbent assay (ELISA),and the content of NO in plasma was measurd by ultraviolet spectrophotometer.Results Compared with model group,the contents of plasma IL-6,TNF-α,TXB2 and CRP in high dose of SMYA(24.3 g/kg) group were significantly decreased,and the contents of NO and 6-K-PGF1α were markedly increased (P<0.05 or P<0.01);the contents of plasma TNF-α in medium and low doses of SMYA (16.2 and 8.1 g/kg) groups were significantly decreased (P<0.01).The  vascular endothelial cells in model group were badly damaged with a large number of  inflammatory cells infiltration,and the  vascular endothelial cells in high dose of SMYA group showed integrity with  little inflammatory cells infiltration.Conclusion SMYA has a good therapeutic effect on rat model of TAO,and the mechanism may be related to anti-inflammatory and  maintaining the balance function of TXA2 and PGI2 in plasma.

Abstract:Objective To investigate the protective effect   of salvianolic acid B  (Sal B) on myocardium of   myocardial infarction model rats and to clarify the molecular mechanism of Sal B intervention in myocardial infarction.Methods The  SD rats were randomly divided into control group,model group,positive drug experiment (AsA and BHT)  groups and observation drug experiment group(Sal B group).The myocardial infarction models of  rats were set up by  coronary artery ligation.The rats in every experiment groups were administered by intraperitoneal injection with the dose of 120 mg?kg-1,the rats in  model group and control group were injected  with the same dose of distilled water for 7 d.Then  the activities of CK,AST and LDH in serum were detected,and  ELISA assay  was used to analyze the expression levels of IL-1β,IL-6 and TNF-α in serum;and  the apoptotic rate  of cardiocytes  in each group was measured,and the clearance rate of free radical was determined by absorption spectrometry.Results  Compared with control group,the activities of serum CK,AST and LDH in model group were increased   obviously（P<0.01）;compared with model group,the activities of serum CK,AST and LDH  in positive drug and Sal B groups were decreased obviously（P<0.05 or P<0.01）.Compared with control group,the levels of serum IL-1β,IL-6 and TNF-α  in model group were increased  obviously(P<0.01）;compared with model group,the levels of serum IL-1β,IL-6 and TNF-α in Sal B group were decreased significantly (P<0.01）;but there was no marked change in AsA and BHT groups（P>0.05）.Compared with control group,the apoptotic rate  of cardiocytes  in model group was increased obviously（P<0.01）;compared with model group,the apoptotic rate of cardiocytes was decreased significantly  in Sal B group （P<0.01）;there was no marked change in AsA and BHT groups（P>0.05）.Different concentrations of Sal B  increased the clearance rate of DPPH free radical  with statistical difference (P<0.05).The clearance rates of free radical in Sal B group were higher than those in AsA and BHT groups when the concentrations of Sal B were 20-100 mg/L（P<0.05 or P<0.01）.Conclusion Sal B has a protective effect on    experimental myocardial infarction   in rats,and  effectively inhibit apoptosis  of cardiocytes.Its mechanism may be related to suppression of immune and inflammatory factor secretion and  free radical scavenging.

Abstract:Objective  To construct the  eukaryotic expression vector of pEGFP-aquaporin-4 and observe the expreesion and location of aquaporin-4 in FRT cells  for its EGFP fusion protein as a reporter by fluorescence microscope and to  provide experimental  basis for further study on  aquaporin-4.Methods The full-length coding sequence of aquaporin-4 was amplified by PCR,and subcloned into pEGFP-N1 for the expression in FRT cells.The FRT cells were transfected with aquaporin-4 using Lipofectamine 2000 Transfection Reagent with different concentrations.The expression of the fusion protein was detected by Western blotting and its location was observed by fluorescence microscope.The osmotic water permeability of the FRT cells was measured using a calcein fluorescence quenching method.Results The results of electrophoresis after restriction enzyme cleavage and sequencing indicated that the EGFP-aquaporin-4 eukaryotic expression vectors were identified.The results of fluorescencemicroscope and Western blotting confirmed the FRT cells expressed aquaporin-4 after EGFP-aquaporin-4 transfection.The osmotic water permeability of the  FRT cells transfeced with  recombinant eukaryotic expression vectors of EGFP-aquaporin-4 was 1.65 times higher than the control. Conclusion The recombinant eukaryotic expression vector of EGFP-aquaporin-4  is established successfully and it is proved that aquaporin-4 can express on FRT plasma membranes by Lipofectamine 2000 transfection.The FRT cells transfeced with aquaporin-4 display high osmotic water transport performance across cell  membranes.

Abstract:Objective To study the influence of anti-sense oligonucleotides(ASODN) of  Survivin on carcinoma cell proliferation by the technique of ASODN.Methods  Survivin-ASODN was synthesized according to Survivin gene sequence.The SMMC-7721 cells were transfected with fluorescence labled Survivin-ASODN.At the same time,blank control,lipofectamine control and SODN control groups were set up.The Survivin gene expression levels were measured by PCR.The proliferation inhibitory rates of Survivin-ASODN transfected cells were analyzed with MTT method.Results The transfection rate reached 60% of fluorescence labled Survivin-ASODN in SMMC-7721 cells.After transfected with ASODN at 100,200,300,400 and 600 nmol?L-1,the Survivin gene expression levels were decreased.[JP2]The inhibitory rates were (3.87±1.67)%，(9.21±2.21)%，(15.21±3.18)%，(21.32±[JP]3.43)%，and (32.62±3.74)%,respectively,at 24 h after transfection. In 300 nmol?L-1 ASODN group,the inhibitory rates were (29.21±4.12)% at 48 h and (44.21±3.32)% at 72 h.Compared with blank control group,lipofectamine  group and SODN group,the inhibitory rates in ASODN group showed significant difference (P＜0.05).Conclusion Survivin-ASODN has inhibitory effect on cell proliferation in dose-dependent and time-dependent manner.

Abstract:Objective To construct the eukaryotic expressing vector of PTEN gene and to express the gene in SCC-4 cell line and to provide theoretical foundation for gene therapy of tongue squamous cell carcinoma.Methods  Human total length of PTEN gene was obtained from HeLa cell line by RT-PCR and was cloned into pMD18-T to analyze the sequence.The correct PTEN was inserted into pEGFP-N1 vector.pEGFP-PTEN was identified by digestion with EcoRⅠ and XhoⅠ.Scc-4 cell line was transfeted by pEGFP-PTEN and the expression of PTEN was observed by fluorescence microscope and Western blotting.Results  A 1 200 bp fragment had been cloned into pEGFP-N1 vector which was further identified by sequencing and NCBI BLAST analysis.pEGFP-PTEN was expressed successfully in SCC-4 cell line.Western blotting showed the expression of PTEN protein in pEGFP-PTEN group was 1.07±0.15,there were significant differences compared with pEGFP-N1 group(0.62±0.11) and blank control group(0.57±0.08) (P<0.05).Conclusion The eukaryotic recombinant vector pEGFP-PTEN is constructed successfully and could be overexpressed in SCC-4 cell line.

Abstract:Objective To construct the eukaryotic expressing vector of PTEN gene and to express the gene in SCC-4 cell line and to provide theoretical foundation for gene therapy of tongue squamous cell carcinoma.Methods  Human total length of PTEN gene was obtained from HeLa cell line by RT-PCR and was cloned into pMD18-T to analyze the sequence.The correct PTEN was inserted into pEGFP-N1 vector.pEGFP-PTEN was identified by digestion with EcoRⅠ and XhoⅠ.Scc-4 cell line was transfeted by pEGFP-PTEN and the expression of PTEN was observed by fluorescence microscope and Western blotting.Results  A 1 200 bp fragment had been cloned into pEGFP-N1 vector which was further identified by sequencing and NCBI BLAST analysis.pEGFP-PTEN was expressed successfully in SCC-4 cell line.Western blotting showed the expression of PTEN protein in pEGFP-PTEN group was 1.07±0.15,there were significant differences compared with pEGFP-N1 group(0.62±0.11) and blank control group(0.57±0.08) (P<0.05).Conclusion The eukaryotic recombinant vector pEGFP-PTEN is constructed successfully and could be overexpressed in SCC-4 cell line.

Objective To study the curative effect of Hawthorn flavones on brain damage in Wistar rats induced by microwave radiation and to clarify the treatment effect of Hawthorn flavones on non-heat effect and heat effect induced by microwave radiation.Methods 36 Wistar rats were randomly divided into normal control group,radiation model group and radiation administration group.The rats  in  the last two groups were given  200 mW.cm^-2  microwave radiation for 5 min.After microwave radiation,the rats in radiation administration group were given 40 mg.kg^-1 Hawthorn flavones once a day,the rats in the other two groups were renderedthe same amount of saline by the same way.The whole process lasted for  14 d.Then the  learning and memory capacities of the rats in 10,11,12,13 and 14 d respectively were detected by Morris Water Maze.The other day after stopping given the Hawthorn flavones,the rats were killed and the brains were removed,then the changes of the brain structures were observed by optical microscope.Results  Compared with normal control group,the target quadrant time,platform residence time,times of crossing platform and times of crossing coverage area of rats in radiation model group and radiation administration group were decreased after 200 mW.cm^-2 microwave radiation (P<0.05);meanwhile the indexes mentioned above in radiation administration group were higher than those in radiation model group (P<0.05).After  200 mW.cm^-2 microwave radiation,the  rat hippocampus and cerebellum neurons appeared nuclear pyknosis and  deep dyeing,and in  radiation administration group  the injury of rat hippocampus neurons was decreased,and restored,without significant changes in cerebellum neurons and blood vessels.Conclusion The learning and memory capacities and the brain structure of the rats can be damaged by microwave radiation with dosage of 200 mW.cm^－2 and Hawthorn flavones has curative effecton brain damage of rats induced by microwave radiation.

Objective To measure the changes of gastric inhibitory polypeptide (GIP) of the  diabetic rats before and after  gastric bypass surgery (GBP) and to discuss the possible mechanism of GBP  in treatment of type 2 diabetes mellitus.Methods The animal models with diabetes mellitus were established in 40 eight weeks old Wistar rats by intraperitoneal injection with STZ，then all the rats were randomly divided into control,diet control,sham operation and operation groups.The rats in control group took food freely,the rats in diet control group were controlled  with  food intake at 15 g/d,the rats in sham operation group  underwent in situ anastomosis after GBP,and the rats in operation group  received GBP.Results Compared with before operation  the fasting and postprandial plasma glucose levels in operation group 4 and 8 weeks after operation were significantly lower than those in the other 3 groups at the corresponding time points(P<0.01);the mean fasting and postprandial serum insulin levels were  significantly higher than those in the other groups at the corresponding time points (P<0.01 ).Compared with before operation,the serum GIP levels in operation group 4 and 8 weeks after operation were   decreased significantly ( P<0.01), and the fasting and postprandial serum GIP levels in operation group were significantly lower than those in the other groups at the corresponding time points (P<0.01).1 week after operation,the average intakes in operation group and sham operation group were decreased and then increased.The average intake in operation group was decreased again from the second postoperative week and was less than those in  sham operation group and control group till the experiment ended (P＜0.05).Compared with before operation,the body mass of the rats in operation group,shame operation group and diet control group were decreased significantly 1 week after operation (P＜0.05).Conclusion The blood glucose and the serum GIP levels of the diabetic rats after GBP are decreased,and its mechanism  may be related to  decreasing  GIP secretion and lifting the peripheral tissue insulin resistance.

Objective To evaluate the stability and histological manifestation at implant-bone interface of the microscrew implants under various loadings and to provide the theoretical basis for the clinical treatment. Methods Microscrew implants(length 7 mm,diameter 1.4 mm) were implanted in the tibae of the rabbits,120 in total,and they were divided into 4 groups,30 in each group.After implantation the implants were loaded immediately with 0,150,300 and 450 g by NiTi coil.The loading period was 20 weeks.After the experiment the rabbits were sacrificed and the displacement of the implants were measured and peri-implant tissues were analyzed histologically.Results The average displacements of the implants in 0 g group,150 g group and 300 g group  were  very small,they were 0.09,0.20, and 0.16 mm; the difference had no significant difference(P>0.05). The average displacement of the implants in 450 g group was decreased (0.60 mm),and the difference of displacement before and after loading was significant(P<0.05).The implant-bone interface in 0 g group  and 150 g group were similar,mainly in the cortical bone.The implant-bone interface in 300 g group was much wider.And the interface in 450 g group  almost contained  all the implants.Conclusion The implants are stable with no displacement under certain amount of loading.Although microscrew is stable,it will have some displacement in some conditions.The implant-bone interface becomes longer with the increasing of loading.

Objective  To study the effect of low intensity ultrasound on osteoclast activity and osteolytic in the mouse skull dissolution model.Methods A total of 30 BALB/c mice were randomly divided into blank control group,grain group and ultrasound group,10 in each group.Improved wear particles were used to replicate mouse skull dissolution models,and the parietal bone samples of mice were obtained 15 d after operation.The number of inflammatory cells and osteolysis area were evaluated.A percentage of bone absorption lacuna area was detected by scanning electron microscope.The proliferation and differentiation of osteoclasts were observed by Tartrate-resistant acid  phosphatase(TRAP)   staining.The expression of osteoprotegerin (OPG) in damage bone tissues was observed by immunohistochemistry staining localization.Results The  HE staining results showed that  compared with blank control group,the exudation of inflammatory cells and osteolysis area  in  grain group and ultrasound group were increased significantly (P<0.01). Compared with grain group,the  osteolysis area in ultrasound group was decreased significantly (P<0.05);but the number of inflammatory cells in  two groups had no significant difference (P>0.05).The scanning electron microscope results showed  that the percentage of bone absorption lacuna area  in  ultrasound group was significantly lower than that in  grain group (P<0.01).The TRAP staining results showed that the number of osteoclasts in ultrasound group was significantly lower than that in grain group (P<0.01). The immunohistochemistry results showed that the number of  OPG positive cells in ultrasound group was significantly higher than those in grain group and blank control group (P<0.05).Conclusion Low intensity ultrasound can inhibit the proliferation and differentiation of osteoclasts in mouse skull dissolution model,and effectively reduce the  bone dissolution  induced by polyethylene wear particles.So the density and strength of bone around joint prosthetic are enhanced,and the incidence of joint mobilization is reduced.

Abstract:Objective To analyze the genotypes of clinical isolates of Mycobacterium tuberculosis in Jilin Province with multiple locus variable numbers of tandem repeats（MLVA）genotyping.Methods Mycobacterium tuberculosis strains were isolated from patient samples in Jilin Province.Genomic DNA was extracted and 12 variable numbers of tandem repeats(VNTR) loci were amplified.The gels were analyzed by Totalab  software and the results were clustered with BioNumerics(6.0).Results Analysis of a total of 110 isolates with the 12 VNTR loci showed great genetic polymorphism in these isolates.Nine of the 12 loci showed the Hunter-Gaston index(HGI) were higher than 0.5.The clustering results showed that total of 6 genotype groups could be defined,of which group 5 was the most represent one,accounting for 34％ of the isolates.The less represented genotypes included group 1,accounting for 15％.Strains of group 5 mainly distributed in Tonghua and Songyuan.Conclusion Mycobacterium tuberculosis isolates in Jilin Province shows great genetic polymorphisms.Different cities have different genotype profiles.

Abstract:Objective To analyze the influence of different doses of atorvastatin on carotid atherosclerosis and ischemic stroke in Chinese and to find safe and effective dose of atorvastatin to prevent ischemic stroke preferably for Chinese population. Methods PubMed，Medline，CNKI，VIP Chinese Science and Technology Journals were used to search randomized controlled trails(RCTs) about  atovastatin on anti-atherosclerosis and stroke prevention   from 1990 to 2011 by computer.The Meta-analysis evaluation software Revman 5.0 was applied，and some indicators were calculated by SPSS 13.0.The changes of intima-media thickness(IMT) and low density lipoprotein-cholesterol(LDL-C),recurrence of ischemic stroke and side effects after treated with different doses of atorvastatin were compared with Meta-analysis. Results 10  and 20 mg atovastatin were widely used by Chinese.Comparative research on megadose atovastatin was still not fully enough.Meta-analysis showed that 20 mg atovastatin was more effective than 10 mg(P＜0.05) in the aspects of anti-atherosclerosis，and the change of IMT in 20 mg atovastatin group was better than 10 mg group,MD=－0.21,95%CI ［－0.25，－0.17］,and the LDL-C level in 20 mg group was lower than that in 10 mg group,MD=0.52，95%CI［0.44，0.61］.At the same time there were no significant differences on adverse effects between two groups.Conclusion 20 mg atovastatin is more better than 10 mg on the changes of IMT,and reduction of LDL-C.There is no difference in adverse effects between 20  and 10 mg atovastatin.

Abstract:Objective  To detect the expression levels of MicroRNA-21 (miR-21) in the colon cancer tissue samples and the cancer-adjacent tissues,and to explorethe relationship between the clinicopathological features and the expression of miR-21.Methods Real-time florescence quantitative PCR（RT-qPCR） was used to detect the relative expression levels of miR-21 in 50 samples including the colon cancer tissue samples and the cancer-adjacent tissues；and the expression changes of miR-21 in two kinds of tissues were analyzed.The relationships between themiR-21 expression levels and the location,depth of invasion and lymph node metastasis of colon cancer were studied.Results The miR-21 relative expression level in colon cancer tissue samples was significantly higher than that in cancer-adjacent tissues(P<0.05);the miR-21 expression level in  right colon cancer tissues was obviously higher than that in left colon cancer tissues (P<0.05);the  miR-21 expression level in Ⅲ stage and Ⅳ stage colon cancer tissues was  obviously higher than  that in Ⅰ stage and Ⅱ stage colon cancer tissues(P< 0.05);the expression levels of MiR - 21 in colon cancer with different depths of invasion had no statistically significant differences(P>0.05);the expression level of miR-21 in  colon cancer tissues with lymph node metastasis was  significantly higher than that in colon cancer tissues without lymph node metastasis (P>0.05).Conclusion The miR-21 expression level is increased  significantlyin colon cancer tissues compared with  cancer-adjacent tissues,and it is  related to the location,the clinical stage and the lymphy node metastasis of colon cancer.

Abstract:Objective To investigate the association between the gene polymorphisms of rs1799939 site of RET exon 11 and papillary thyroid carcinoma(PTC),and to provide theoretical basis for genetic mechanism of PTC.Methods A case-control 　study was used in this research.100 cases of PTC were selected as case group,100 healthy people matched in age and sex were selected as control group.PCR-RELP analysis was employed to detect the genotypes of the two groups，statistical analysis software SPSS 13.0 was used to analyze the association between the gene polymorphisms of rs1799939 site and PTC.Results The distribution of genotypic frequency of rs1799939 site didn’t deviate from Hardy-Weinberg equilibrium in both case group and control group (P>0.05).The distribution of genotypic and allelic frequencies at rs1799939 site showed no statistical significance between case group and control group (P>0.05).The distribution of genotypic and allelic frequencies between different genders in case group showed no statistical significance either (P>0.05).Conclusion The polymorphism of rs1799939 site in the RET gene may be not associated with the occurrence of PTC.

Abstract:Objective To explore the expression of tumor stem cells marker aldehyde dehydrogenase 1(ALDH1) in noninvasive bladder cancer tissue and to investigate the relationship between tumor stem cells and the biological characters of bladder cancer.Methods The expressions of ALDH1 in 118 cases of noninvasive carcinoma specimens and 30 cases of normal bladder tissue surrounding cancer weredetected by immunohistochemistry and analyzed by combining with clinical pathology and prognosis data.Results The positive expression rates of ALDH1 were 24.58% and 6.67% in specimens of cancer and normal bladder specimens,respectively,and the difference between them was statistically significant(P<0.05).The positive expression rate of ALDH1 in high-grade group(36.59%,15/41) was higher than that in low-grade group(18.18%,14/77)(P<0.05).The positive expression rate of ALDH1 in pT1 stage(33.96%,18/53) was higher than that in pTa stage(16.92%,11/65)(P<0.05).The positive expression rate of ALDH1 in recurrent group(37.50%,12/32) was higher than that in primary tumor group(19.77%,17/86)(P<0.05).The ALDH1 expressionwas not related to gender,age and tumor size of patients with noninvasive bladder cancer(P>0.05).During a 4-52 months follow-up period,the tumor-free survival rates in ALDH1-positive group and ALDH1-negative group were 55.17% and 61.80%,respectively.Kaplan-Meier analysis and  Log-rank test showed that the differences of cumulative tumor-free survival rate was statistically significant between ALDH1-positive group and  ALDH1-negative group(P<0.05).Conclusion The ALDH1 expressionis related to the pathological grades,stages and recurrence of bladder cancer.Thetumor stem cells may play a role in the tumorigenesis and recurrence of noninvasive bladder cancer.

Abstract:Objective To investigate the relevance between plasma content,plasma protein molecular forms and promoter region polymorphisms of mannose binding lectin(MBL) and ischemic cerebrovascular disease (ICVD).Methods The anti-coagulated blood of patients with ICVD (ICVD group,81 cases) and healthy people (control group,60 cases) was collected.The plasma level of MBL was measured by ELISA and the plasma MBL protein oligomeric forms were determined by Western blotting.The promoter region polymorphisms of MBL gene were analyzed by sequence specificprimers PCR (SSP-PCR).  Results  The plasma content of MBL in ICVD group (3 372.18  μg/L±660.90  μg/L)  was higher than that in control group (2 065.29   μg/L±195.67 μg/L) (P<0.01).The bands represented single MBL peptide sub-unit (approximately 36 000) and oligomeric forms (approximately 130 000 and 250 000) were deeper in ICVD group than those in control group.The constituent ratios of haplotype and genotype of MBL promoter region in ICVD group were significantly different from those in control group.There were  significant differences in the frequencies of haplotype HYP and LXP between ICVD patients(50.62%,6.79%) and controls(34.17%,20.83%) (P<0.05).HXP/HYP and HXP/LYQ genotypes were found only in ICVD patients,meanwhile,HXP/LXP and HYP/LXP genotypes only in controls.ICVD patients  had significantly lower frequency of genotype LYQ/LYQ(4.94%) compared with controls (15.00%)(P<0.05).Conclusion The plasma content,plasma protein molecular forms and constituent ratios of haplotype and genotype of MBL promoter region in ICVD patients are all different from those in healthy people,so MBL may be involved in the development and progression of ICVD.

Abstract:Objective  To study the electrochemical corrosion of Ni-Cr alloy,Co-Cr alloy and pure titanium(Ti) in Cola,and to provide  basis for choice of clinical materials.Methods Ni-Cr alloy,Co-Cr alloy and pure Ti were divided into 3 groups.The classic three-electrode system was used to measure thecorrosion potential (Ecorr) and polarization curves,the polarization resistance (Rp),and corrosion current density (Icorr)  in three groups in artificial saliva and Cola,and the results were analyzed by statistics.Results  The values of Ecorr and Rp of Ni-Cr alloy,Co-Cr alloy and pure Ti in Cola were lower than those in artificial saliva(P<0.05),and the values of Icorr of them in Cola were higher than those in artificial saliva(P<0.05). In Cola,the values of Ecorr and Rp were Ni-Cr alloy＜Co-Cr alloy<pure Ti（P<0.05）,and the values of Icorr were Ni-Cr alloy＞Co-Cr alloy＞pure Ti（P<0.05）.Conclusion All of three kinds of metal materials have faster corrosion rate and poorer corrosion resistance in Cola than in artificial saliva.The corrosion rate of pure Ti in Cola is the slowest and the corrosion resistance is the best,while the corrosion rates  of both  Ni-Cr andCo-Cr in Cola are faster.

Objective  To investigate the effects of different target concentrations of propofol on mRNA expressions of pro-inflammatory cytokines as interleuk
in-1（IL-1）,interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in patients underwent cardiopulmonary bypass (CPB) surgery,and to explore the ideal concentration of propofol in Chinese people for clinical use. Methods Sixty patients underwent CPB were randomly assigned into three groups (n=20) according to the different target concentrations:group A,2 mg?L-1; group B,3 mg?L-1;group C,4 mg.L^-1.The blood samples were taken at the time of induction(T1),right after CPB(T2),30 min after CPB(T3),60 min after CPB(T4) and 3 h after CPB(T5).The mRNA expressions of cytokines were detected by RT-PCR.Results  The levels of the serum cytokine expressions of the patients in three groups showed no difference before C
PB,while the levels of all cytokines rushed up after CPB and went up at peak between 30-60 min after CPB,which had great differences at T1,T2,T3 and
T4 compared with T0.Compared with group A,the levels of all cytokines in group B and group C  were significantly decreased(P<0.05).However,the expression levels of cytokines  had no difference between group B and group C. Conclusion Propofol can inhibit the expressions of serum pro-inflammatory cytokines,including IL-1,IL-6 and TNF-α,in patients underwent CPB surgery;therefore inhibit inflammatory reaction.The recom
mended target concentration of propofol would be 3 mg.L^-1 in CPB surgery. 

Objective To observe the effect of phacoemulsification surgery on tear film of the patients with different ages and to investigate the clinical significa
nces.Methods 154 cases(154 eyes) with cataract accepted phacoemulsification combined with intraocular lens implantation were chosen and divided into three groups according to different ages:group A,30 eyes of the patients aged <60 years;group B,68 eyes of the patients aged 60-75 years;group C,56 eyes of the patients aged　>75 years.Schirmer Ⅰ test,tear break-up time (BUT),corneal fluorescein staining and dry eye symptom score were compared among three groups.Results 7 d after operation,the dry eye symptom score in group A was higher than those in groups B and C,there were significant differences（P<0.05 or P<0.01）;1 and 3 months after operation,the scores had no statistically significant differences between three groups.7 d after operation,the Schirmer Ⅰ test value in group C was lower than those in group A and B（P<0.01）.7 d after operation,the BUT in group A was longer than those in
 group B and C,there were significant differences（P<0.01）.1 and 3 months after operation,there were statistically significant differences between group C and group A,B（P<0.05 or P<0.01）.The BUT of the patients in group C didn’t recover to preoperative level 3 months after operation（P<0.01）.7 d and 1 month after operation,the corneal fluorescein staining score in group A was lower than those in group B and C,there were significant differences（P<0.05 or P<0.01）;3 months after operation,there were no statistically significant differences between three groups.Conclusion The injury degree and recovery time of ocular surface of the patients with different ages after phacoemulsification surgery are different;the injury of ocular surface after operation in elder patien
ts is more serious and the recovery time is longer  than the younger patients.

Objective To observe the curative effect of different doses of radiotherapy combined with cisplatin weekly therapy in the treatment of advanced cervi
cal cancer,and to explore better treatment options. Methods 135 cases of stage  ⅡB－ⅣA cervical cancer patients collected in Jilin Provincal Tumor Hospital
 from January 2007 to June 2008 met   the inclusion criteria were randomly divided into radiotherapy group(n=45,radiotherapy alone) and
concurrent chemoradiotherapy group(n=90),including 20 mg/m^-2 low dose group(n=45) and 40 mg/m^-2 standard dose group(n=45).
The 3-year survival rates and the incidence of related toxic reactions  in radiotherapy group and concurrent chemoradiotherapy group were compar
ed.At the same time the 3-year survival rates and the incidence of related  toxic reactions in 20  mg/m^-2 low dose group and 40  mg/m^-2 stand
ard dose group were compared.Results The median follow-up time was 42 (6-51) months.The 3-year survival rates  in  radiotherapy group and
  concurrent chemoradiotherapy  group  were 68.9% and 83.3%,the difference was statistically significant (χ2 = 3.858,P<0.05).The in
cidence of Ⅲ-Ⅳ acute toxic reactions in  radiotherapy  group was lower than that in  concurrent chemoradiotherapy group (χ2 = 4.072,P<0.05).The 3-year survival rates in  20 mg/m^-2 low dose group and 40  mg?m-2 standard dose group  were 82.2% and 84.4%,there was no significant difference between  two groups (χ² =0.090,P>0.05).The incidence of acute toxic reactions  in 20 mg/m^-2 low dose group was significantly lower than that in  40 mg?m-2 standard
 dose group(χ² =3.920,P<0.05).Conclusion Compared with radiotherapy alone,concurrent chemoradiotherapy can  significantly improve the efficacy of  ⅡB－ⅣA cervical carcinoma.In the concurrent chemoradiotherapy,compared with  40 mg?m-2 standard dose cisplatin,20 mg/m^-2 low dose cisplatin  doesn’t  reduce the effect of radiotherapy in patients,but  reduce the incidence of Ⅲ-Ⅳ stage acute toxic reactions  in patients.


Objective To discuss the curative effect of cannulated compression screws in treating the fresh scaphoid fracture without displacement,and to clarify the advantages of palmar miniature incision in treatment of fresh scaphoid fractures without displacement. Methods 14 patients with fresh scaphoid fracturewithout displacement were treated with AO　3.0 mm  cannulated compression screws internal fixation.The average age of the patients was 32 years old.The patients were classified by Krimmer pattern into 2 types: 6 patients were A1 type and the other 8 patients were A2 type.All the patients underwent internal fixation with the palmar miniature incision procedure.The fracture healing situation was observed by postoperation reexamination of X-rays.The wrist joints recovery and functional status were evaluated by Krimmer score standard.Results the average follow-up time was 12 months.14 patients with scaphoid fracture were cured with favorable function,11 got the best function,2 got the better,and 1 got good.The range of motion of the wrist was 50°-165° with an average of (120±25)°.The cubit radial deviation was 28°-81° with an average of (61.0±10.4)°.The grip strength was 20-65 kg with an average of (50.0±6.7) kg.Conclusion Cannulated compression screws by the palmar miniature incision procedure has got a good effect in treatment of fresh scaphoid fracture without displacement.

Objective To  detect the refractive status of the 40 to 49 years old outpatients by cycloplegic refraction and the correction and to explore the method to improve the visual fatigue and symptoms of dry eye. Methods 30 visual  fatigue outpatients (60 eyes) with complaints of sore eye,dry eye,blurred vision，aged  40 to 49 years old  (average 44.43 years) were selected,11 patients were male and 19 patients were female;the other diseases such  as glaucoma,cataract,ocular fundus were ruled out after routine eye examination. The patients had no surgical and wearing myopia,hyperopia and astigmatism glasses history. Mydriasis refraction was porformed after conventional optometry,and the SchirmerⅠtest,tear film break-up time(BUT) detection and corneal fluorescent staining were performed simultaneously. 43 eyes of 30 patients had a visual acuity of ≥ 0.8,and 17 eyes were between 0.4 and 0.6. Results Among all patients,37 eyes presented －0.25DS to －1.25DS with conventional refraction,36 eyes showed +0.50DS to +1.50DS after dilation;22 eyes presented 0.00DS to +1.25DS before dilation and 21 eyes showed+0.50DS to +1.50DS after dilation;27 eyes were diagnosed as moderately dry eye and 33 eyes were slightly dry eye. Conclusion Patients aged 40-49 years old with normal or close to normal results in conventional inspection still have considerable accommodation ability. There may exist hyperopia or hyperopic astigmatism in the patients with visual fatigue,dry eye and even a special face (frown). The examination results often exist error that is easily overlooked before dilation. The sore eye,dry eye,blurred vision and other visual fatigue symptoms in 30 patients (60 eyes) are significantly improved after optometry and optician. Therefore optometry after mydriasis and wearing glasses should be noted in the treatment of dry eye,that is the fundamental solution to relieve the symptoms of ocular discomfort completely.

Abstract:Objective To explore the method of  minimal-single-operating-port video assisted thoracic surgery (VATS) in treatment of spontaneous pneumothorax and to clarify  its clinical advantages. Methods Retrospective analysis of 96 patients with spontaneous pneumothorax  underwent VATS was performed.The patients were divided into two groups according to different  medical treatment groups and the different surgical method:minimal-single-operating-port VATS(MVATS) group  including 23 cases treated by minimal-single-operating-port VATS approach and conventional VATS group including 73 cases treated by two-operating-port VATS or conventional single-operating-port VATS. The mean operation time,mean intraoperative blood loss and average postoperative hospital stay,average postoperative chest tube duration and postoperative pain grades of the patients in two groups were analyzed and compared. Results The mean intraoperative blood loss,average postoperative hospital stay,and average postoperative chest tube duration had no significant difference between MVATS group and VATS group（P>0.05）;however,the incidence of  postoperative pain in MVTS group was significantly lower than that in VATS group (P<0.05). Conclusion Compared with two-operating-port VATS and conventional single-operating-port VATS,minimal-single-operating-port VATS has the similar clinical efficacy and better cosmetic result,and reduces the postoperative pain effectively in treatment of spontaneous pneumothorax.

Abstract:Objective To explore the surgical method of bony mallet finger  and to evaluate the clinical effect of fixed needle extrusion in treatment of bony mallet finger.Methods 28 patients with bony mallet finger were treated with kirschner needle extrusion to  reset the fracture fragments and fix　the fracture.The flexion or extension of  distal interphalangeal joint (DIP),fracture healing and postoperative complications of the patients were observed.Results 27 patients were followed-up for 14 months.Crawford assessment standard was used to assess the efficacy.Before operation,the DIP extension limitation of 10 °-25 ° or obvious  flexion  obstacles existed in 8 cases (29.6%);the DIP extension limited by greater than 25 ° or the presence of persistent pain existed in 20 patients (70.4%).After operation,the fully active flexion and extension of DIP without pain in resting or action existed in 21 cases (77.8%);the  full flexion of DIP,extension limitation from 0 ° to 10 °without pain in  resting state  existed in 6 cases (22.2%).All fractures in the patients healed,and the joint face apposition was good without osteomyelitis and wound complications such as infection,necrosis and recurrence of mallet finger deformity.Conclusion Kirschner needle extrusion  fixation method  for the treatment of bony mallet finger is a simple method with satisfatory curative effect.  

Abstract:Objective  To locate the optic canal precisely by measuring related distances and angles between optic canal and certain landmarks. Methods 120 CT images from clinical database were chosen for measurement.The  line (L1) of the nasal bone tip (P1) and the middle point of tuberculum sellae (P2)  in centre sagittal plane were set as a baseline.The plane went through L1 and perpendicular to centre sagittal plane (L2) was set as measurement plane.The parameters were the medial canal wall length (D1),the distance between nasal bone tip (P1) and orbit end of optic canal (P3 ) (D2),the distance between P1 and cranium end of optic canal (P4) (D3),the distance between P3 and L2 (D4),the distance between P4 and L2 (D5),the angle between P1P3 and L2 (A1),and the angle between P1P4 and L2 (A2).Results  D1 was (10.51±1.07) mm on the left,(10.64±1.10) mm on the right,statistical difference was found 〖JP3〗between the two sides (P<0.05). D2 was (66.68±5.99) mm on the left,(66.81±〖JP〗5.97) mm on the right,there was no statistical difference between the two sides (P>0.05). D3 was (72.82±6.33)  mm on the left,(73.04±6.33)mm on the right,statistical difference was found between the two sides(P<0.05).D4 was (13.96±1.43) mm on the left,  (14.16±1.53) mm on the right,there was no statistical difference between the two sides (P>0.05). D5 was (6.65±1.25) mm on the left,(6.58±1.41) mm on the right,there was no statistical difference between the two sides (P>0.05). A1 was (12.26±1.63)°,and  A2 was (5.28±1.13)°.Conclusion  The parameters above can help to  locate the optic canal precisely in the measurement plane and provide reference for the decompression surgery.It also makes the surgery safer.

Objective  To establish stable and reliable rat C6 glioma model,and to perform MRI dynamic observation and pathomorphological observation in model animal brain,and to provide experimental basis for pharmaceutical research on　anti-glioma drugs.Methods The C6 glioma cells were cultured and 20 μL cultural fluid containing 1×10⁶ C6 cells was sterotactically implanted into the left caudate nuclei in 10 male Wistar rats,respectively.The changes in the behavior of the rats after implantation were observed and recorded.MRI dynamic scanning was performed in 10 rats  2,3 and 4 weeks after implantation and the brain tissues were taken for general and pathological examination when the 10 rats were naturally dead.The survival period of tumor-bearing rats was calculated.Results 2 weeks after implantation the rats showed decreased activities and food intake,fur lackluster,and conjunctival congestion a
nd so on;3 weeks later,some rats appeared nerve symptoms such as body twitch,body hemiplegy,body distortion,rotation and so on.All the 10 rats died in 8 -
30 d.The median surrival period of the tumor-bearing rats was 18 d,the average survival period was (18.3±7.3) d.The pathological examination showed that
the tumor cells were arranged irregularly and closely and karyokinesis was easy to see;tumor vascular tissue proliferation and tumor invasive growth into
surrounding noral tissues were found.The expression of glial fibrillary acidic protein(GFAP) was positive in the tumors.
Conclusion  A stable animal model of  intracranial glioma is sucessfully established by  stereotactic implantation of C6 cells into the rat caudate nucleus.The results of  MRI 〖JP2〗dynamic observation and pathohistological observation on the model animal brain tissue.can provide experimental
basis for selecting the appropriate time window to perform the pharmaceutical research on anti-glioma drugs.

Objective To  evaluate the safety and effectiveness  of hysteroscope in diagnosis and treatment of retained placenta accreta by comparing the clinical effects of two methods of hysteroscope and medicine  in treatment of  placenta accreta.Methods A retrospective analysis  of fourty-seven patients with retained placenta accreta  including  7 cases of placenta accreta,38 cases of placenta increta,2 cases of  placenta percreta.There were 7 cases in first trimester,19 cases in second trimester,and 21 cases in last trimester.38 patients with placenta increta were treated with hysteroscopic resectin(n=20) and mifeprist
one+methotrexate(n=18),respectively.The clinical effects of two methods were compared by detecting the B ultrasound negative rate,negative rate of human chorionic gonadotropin(HCG) and 3-month normal menstruation rate.Results The risk factors of placenta accreta in various trimesters were basically c
onsistent. Among them curretage was the first risk factor of   placenta accrete,65.9%(31/47)；the second was cesarean,34.0%(16/47).85.7%（6/7） placenta accrete was placenta accrete in first trimester； 92.5%（37/40） was placenta increta in second and third trimesters. 27 cases were cured by
hysteroscopic resection controlled by B ultrasound.A simultaneous laparoscopy was performed in 1 case.19 cases were completed in one operation,and 1 case underwent two operations.There was no complication.The B ultrasound negative rate and 3-month normal menstruation rate in hysteroscope group(100%,100%) were higher than those in medicine group(64.7%，58.8%)(P<0.05).There was no significant difference of negative rate of HCG between two groups(P>0.05).Conclusion The treatment of combined hysteroscope and B ultrasound is more effective than medicine.

Objective  To study the changes of peripheral blood cells of the employees who accept a long-term extremely low frequency electromagnetic radiation,
and to investigate the effects of the extremely low frequency electromagnetic radiation on the peripheral blood cells.
Methods The employees accept extremely low frequency electromagnetic radiation were chosed as exposure groups,the worker
s who exposed to the low level of electromagnetic radiation were used as  low exposure group (n=123),and those who exposed to the high level radiation a
s  high exposure group(n=229),another workers who did not contact with extremely low frequency electromagnetic radiation were chosed as control group (n=212).The electromagnetic field intensity was detected by EFA-300 Field Analyzer,the workshop noise intensity was detected by AWA5610D integrating sound level meter,and the number of peripheral blood cells was tested by automated hematology analyzer.
Results Compared with control group,the mean corpuscular volume (MCV) and the number of  white blood cells (WBC) in low exposure group were significantly increased (P<0.05);the  MCV,WBC,hematocrit (Hct),and lymphocyte (LC) in high exposure group were markedly
increased (P<0.05),but the mean platelet volume (MPV) in high exposure group was decreased(P<0.05).Conclusion Extremely low frequency electromagnetic radiation might affect the peripheral blood cells of employees,even cause the changes of blood system. 

Objective To explore the difference in levels of mental health in teachers of medical colleges of different kinds of self-esteem and to clarify the
relationship between them.Methods Stratified sampling method was used,and 665 teachers in medical colleges were tested by Self-Esteem Scale (SES),Marlowe-Crowne Social Desirability Scale (MCSD) and  Mental Health Scale (MHS) for the levels of self-esteem and
mental health.Results The  scores of self-esteem and social desirability  were both significantly positively related to  the thoal score of  mental he
alth（r=0.67，P<0.01；r=0.50，P<0.01）;the scores of mental health in different kinds of self-esteem were all significantly different in total scores,dimensions and factors（P<0.01）;the total score of mental health of the high self-esteem was significantly higher
 than that of the low self-esteem（P<0.01）;the total score of mental health of the defensive high self-esteem was significantly higher than   that of the security high self-esteem,too（P<0.01）.Conclusion To medical teachers,self-esteem is significantly positively related to mental health.High self-esteem,especially with high social desirability contributes to mental health.

Objective To understand the contents of Ca,Fe,Zn and Cu in hair of primary and secondary students in Jingyu county  and Dongliao of
Jilin Province and to provide basis for guiding reasonable diet and preventing the occurrence of mineral deficiency.
Methods Stratified cluster sampling method was used  to extract   675 primary and secondary school students and the
 Ca,Fe,Zn and Cu contents in the hair were detected  by ICP-MS method;the contents and deficiency rates of the four kinds of minerals in the hair of 6
75  primary and secondary school students with different genders and different ages were compared;and the relationships between Ca,Fe,Zn,Cu,and the correlations of the four kinds of minerals  with the  morphological indexes were analyzed.Epidata 3.0 was used to establish the database,and SPSS 13.0 statistical software was used for   statistical analysis.Results The Ca deficiency rate of 675 primary and secondary students was the highest (32.9%,222
/675),followed by Fe (22.4%,151/675),Zn (16.6%,112/675) and Cu (0.6%,4/675).The  distribution of Ca,Fe and Zn contents in the hair of the students with different genders had significant differences (P<0.05),and the girls’, were higher than the boys’,but  the distribution of Cu contents   had no significant difference (P>0.05);the distribution of Ca,Fe,Zn contents  between different ages groups had significant differences (P<0.05),and the deficiency  rates of Ca and Zn in 8 to 10 age group were the highest;but the distribution  of content Cu  had  no statistically significant difference (P> 0.05).The age had positive correlation with   Ca,Fe,Zn and Cu contents(rs = 0.129,rs = 0.075,rs =0.204,rs = 0.087,all P<0.05);and  the Fe content had positive correlations with Zn and Cu contents(rs = 0.576,rs = 0.149,all P<0.05);and the Ca  and Zn contents had positive correlations with the height,weight,chest circumference and sitting height of the students(P<0.05).Conclusion The deficiency rates of Ca and Fe in 675 primary and secondary students are higher;the contents of Ca,Fe
,Zn,and Cu  are important factors that affect the growth and development of children,so  reasonable diet and appropriate supplement of Ca and Fe sh
ould be performed.


Objective  To establish an indirect sandwich enzyme-linked immunesorbent assay (ELISA) kit for detecting serum level of mucin 1(MUC1) protein and to
explore its application in diagnosis of lung cancer,and to reveal the value of MUC1 protein  detection in clinic.Methods With rabbit anti-human MUC1 monoclonal antibodies and rat anti-MUC1 polyclonal antibodies as coating antibodies and testing antibodies,respectively,a new ELISA method was developed in this study,and the standard curve was gotten.Then,the serum MUC1 levels in the peripheral blood of 48 cases with lung cancer,7 cases with lung benign diseases,and 20 healthy subjects were detected with indirect ELISA.The optimal cut-off value,the specificity,the sensitivity and the Youden index were determined with ROC curve analysis.At the same time,the serum levels of MUC1 detected by CA15-3 commercial kits were analyzed.
Results Based on indirect sandwich ELISA,the cut-off value for serum MUC1 was 1.98  μg?L-1,the sensitivity was 62.5%,the specific
ity was 100% and the Youden index was 0.6250.While based on the CA15-3 kit,the sensitivity was 18.75%,the specificity was 100% and the Youden index was 0.187 5.Conclusion The indirect ELISA kit is superior to the CA15-3 kit in detecting MUC1 in lung cancer .

Objective To analyze the distribution and genotyping of aminoglycoside modifying enzyme genes in gram-negative bacilli from some hospitals in Changchun area and to provide a reasonable basis for the application of antibiotics. Methods Amikacin-resistant and gentamicin-resistant strains were screened using agar dilution tests,and the aminoglycoside modifying enzyme genes were amplified by PCR and analyzed by sequencing.
Results The resistant rates of 69 strains of gram-negative bacilli to  amikacin and gentamicin were 44.9% and 87.0%,respectively.63 strains of gram-negative bacilli (91.3%) were aminoglycoside modifying enzyme-positive,the genotypes of aminoglycoside modifying enzymes included aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb,ant(3")-Ⅰ,and ant(2")-Ⅰ,with positive rates of 4.3%,81.2%,43.5%,33.3%,and 13.0%,respectively.But the aac(6′)-Ⅱ　was not found.
Conclusion The genotypes of aminoglycoside modifying enzymes of gram-negative bacilli in local area are aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb,ant(3")-Ⅰ,and ant(2")-Ⅰ,and the most common of aminoglycoside modifying enzyme genes is aac(3)-Ⅱ.Reasonable application of aminoglycosid
e antibiotics to reduce bacterial resistance has great significance.

Objective  To investigate the application  value  of nucleic acid isothermal amplification-immunochromatographic test in the diagnosis of brucellosis
and to provide experimental basis for clinical application of nucleic acid test.Methods The brucella nucleic acid was extracted from blood and serum samples, and  isothermal amplification technique was applied to amplify nucleic acid and  immunochromatographic test device was used to interpret the results of nucleic acid amplification directly.Tube agglutination test (SAT) was used as immunological tests.The pairing Chi-square test was performed  in the data analyses.Results The brucella nucleic acid was extracted from the  whole blood and serum of 29 cases,the amplification rate of whole blood nucleic acid was 55.2%,the amplification rate of serum nucleic acid was 23.1%,and there was significant difference (P<0.05).The nucleic acid was extracted from the whole blood of 190 cases in different course,using SAT diagnostic technique,the cases were divided into positive and negative treatment
 populations,to these people,the agreement rate of the nucleic acid test results and SAT test results was 57.9%. 93 cases who saw the  doctor at the first time were divided into positive and negative treatment populations with SAT diagnostic technique,to these people,the agreement rate of the nucleic acid test results and SAT test results was 63.4%.Conclusion As a reference of laboratory-assisted diagnosis and treatment,nucleic acid isothermal amplification- immunochromatographic test has higher clinical application value for acute early brucellosis cases,but for differential diagnosis has no reference value for  brucellosis patients and non-brucellosis patients.


Objective  To detect the ability of gallic acid (GA) to  restore Fe3+ in vitro,and  explore its antioxidant activity in vitro an
d to compare it with recognized antioxidant ascorbic acid (Vc),and to provide experimental basis for antioxident application of GA.
Methods Different concentrations of GA (0.04,0.08,0.12 and 0.16 g/L^-1) and Vc (0.04,0.08,0.12 and 0.16 g/L^-1) were used in the phenant
hroline-Fe2+ system to  occurre Fenton reaction and the potassium ferricyanide-Fe3+ system to occurre Fe3+ restore reaction.The A values of the solution after reaction were detected with  UV spectrophotometer. SPSS 17.0 package and two-way ANOVA were used to analyze the relationship between the A values and concentrations of GA and Vc solution.Results  In the phenanthroline-Fe2+ system，the A value of  GA solution was larger than that of Vc at the same concentration(P<0.05).Compared with 0.04 g/L^-1 GA group,the A values in 0.08，0.12，and 0.16 GA groups were gradually increased(P<0.05);and ethanol influenced the A value of the phenanthroline-Fe2+ system.In potassium ferricyanide-Fe3+ system,the Fe3+ reduction ability of GA was stronger than Vc when their concentrations were  0.04 g?L-1;the abilities were weaker  in the other concentrations.When the concentration of HCl was higher than 0.84× 10－2mol/L^－1,it had  great influence in the A value of potassium ferricyanide-Fe3+ system.Conclusion Compared with Vc,GA has a stronger Fe3+ restoring capacity under certain conditions in vitro.
