Abstract:

To explore the effect of ［5-nitro-2-(3-phenylpropylamino)-benzoic acid］NPPB，a chloride channel blocker,on the growth of human glioma SHG-44 cells and to clarify  the potential mechanism of proliferation inhibition and apoptosis induction of NPPB on SHG-44 cells.Methods The glioma SHG-44 cells were cultured and divided into control and 50,100 and 200   μmol•L^-1 NPPB groups,and the influence of NPPB in proliferation of SHG-44 cells was　measured by MTT assay.The cell cycle and apoptotic rate were analyzed by Cell Analysis Machine.Results Compared with control group,the proliferation of SHG-44 cells was inhibited after treated with 50   μmol•L^-1 NPPB for 48 h (P<0.05),the proliferation of SHG-44 cells was improved after treated with 200   μmol•L^-1 NPPB for 3 h (P<0.05) and the proliferation of SHG-44 cells were inhibited significantly after treated with 100 and 200   μmol•L^-1 NPPB for 24 and 48 h (P<0.01).The detection  results of cell cycle showed that compared with control group,the percentage  of SHG-44 cells  in G1 phase was decreased and the percentage of SHG-44 cells in G2/M phase was increased(P<0.01) in 50   μmol•L^-1 NPPB group 24 h after treatment;Part of SHG-44  cells was arrested in G1 phase in 100 and 200   μmol•L^-1 NPPB groups 24 h after treatment（P<0.01）.The detection  results of apoptosis  showed that the apoptotic rates of SHG-44 cells were increased to 24.64% and 41.85% after treated with 100 and 200   μmol•L^-1 NPPB for 24 h, respectively,compared with control group (P<0.01).Conclusion High doses of NPPB can inhibit the proliferation and induce apoptosis of SHG-44 glioma cells,which indicates that chloride channels may play essential roles in the proliferation and apoptosis of human glioma SHG-44 cells.

Key words: chloride channel, glioma, cell proliferation, apoptosis