Abstract:

To observe the effect of the eukaryotic translation initiation factor 4E (eIF4E) targeted small interfering RNA (siRNA) on the eIF4E expression in human laryngeal Hep-2 cells,and to clarify the induction effect on apoptosis of Hep-2 cells and its mechanism.Methods The siRNA-eIF4E was transfected into human laryngeal carcinoma Hep-2 cells (siRNA-eIF4E group) with LipofectamineTM 2000,at the same time blank control group and empty plasmid group were set up.The inhibitory rate of cell proliferation in each group was detected by MTT.The changes of mitochondrial membrane potentials of the cells were observed by Rhodamine dyes.Apoptosis was detected by TUNUL staining,and the transcription and protein expression levels  of apoptosis related genes were analyzed by RT-PCR and Western blotting method.Results Compared with blank control group and empty plasmid group,the transcription and protein expression levels of eIF4E in Hep-2 cells in siRNA-eIF4E group were significantly decreased(P<0.01),the survival rate of Hep-2 cells was  decreased (P<0.05),the mitochondrial membrane potential of the cells was decreased (P<0.05),the apoptotic rate was increased (P<0.05),and the expression levels of apoptosis related genes Bim,Bid and Caspase3 were significantly up-regulated (P<0.05).Conclusion siRNA-eIF4E can inhibit the proliferation of human laryngeal carcinoma  Hep-2 cells in vitro,and its mechanism may be related to the activation of mitochondrial apoptosis pathway.

Key words: small interfering RNA, eukaryotic translation initiation factor 4E, laryngeal neoplasms, Hep-2 cells, apoptosis