Abstract:

Abstract:Objective  To investigate the intervene effect of JieDu TongLuo BaoShen capsule (BaoShen capsule) on the  expressions of  adiponectin protein and monocyte chemoattractant protein-1 (MCP-1) in kidney tissue of  type 2 diabetic rats,and to clarify the renoprotective effect of BaoShen capsule and its mechanism.Methods Sixty-five male Wistar rats were divided randomly into normal control group (control,10 rats,fed standard rat chow),high-fat diet feeding group(High-fat control,10 rats,fed high-fat diet) and diabetic group(45 rats,fed high-fat diet).Four weeks later,type 2 diabetes was induced in the  rats in diabetic group by intraperitoneal injection of a low dose of streptozotocin.After modeling they were again randomly divided into untreated diabetic model group(DM,15 rats),BaoShen capsule(1.4 g/kg) treatment  group (BaoShen capsule,15 rats) and rosiglitazone combined with benazepril treatment  group (Positive control,15 rats),and treated for 12 successive weeks while continuing on the high-fat diet.Twelve weeks later,the blood glucose (BG),serum creatinine (Scr) and blood urea nitrogen(BUN) levels,urinary microalbumin excretion rate (UAE) were measured.The protein expression of  adiponectin in kidney tissue was determined using immunohistochemistry.Results Compared with control group,the  blood glucose,serum Scr and BUN levels,and UAE of the  rats in BaoShen capsule treatment group were significantly decreased(P<0.01);the serum adiponectin level  and the protein expression of  adiponectin in kidney tissue of the rats in DM group were markedly reduced   compared with control group(P<0.01),and the protein expression of  MCP-1 in kidney tissue was obviously elevated (P<0.01). Compared with DM group,the serum adiponectin level  and protein expression level of  adiponectin in kidney tissue in Baoshen capsule group were increased by 1.5 and 3 folds (P<0.01),while the MCP-1 protein expression level in kidney tissue was decreased by 55% (P<0.01).Correlation analysis indicated that the  serum adiponectin levels were negatively correlated with UAE(r=－0.466,P<0.01),Scr (r=－0.563,P<0.01),BUN (r=－0.512,P<0.01) levels and  MCP-1 protein expression level in kidney tissue (r=0.526,P<0.01).Conclusion BaoShen capsule treatment can increase the serum adiponectin level,up-regulates the adiponectin level in kidney tissue and and down-regulate the MCP-1 protein expression level in kidney tissue in type 2 diabetic rats,that is linked to renoprotective capacity of BaoShen capsule.

Key words: JieDu TongLuo BaoShen capsule, type 2 diabetes mellitus, diabetic nephropathy, adiponectin, monocyte chemoattractant protein-1