茵陈提取物对糖尿病大鼠肾组织中PTEN蛋白表达的影响及其肾脏保护作用

doi:10.13481/j.1671-587x.20190406

Abstract

Abstract: Objective:To investigate the effect of herba artemisiae capillaris extracts (HACE) on the expression of phosphatase and tensin homologue deleted chromatosome 10 (PTEN) in the kidney tissue of the diabetic rats, and to clarify the pharmacological mechanism of protective effect of HACE on the kidney of the diabetic rats. Methods:Thirty Wistar rats were randomly divided into control group (n=6), model group (n=12) and HACE group (n=12).The rats in HACE group were intragastrically administrated with HACE(5 g·kg^-1) one time every day,and the rats in control group and model group were intragastrically administrated with the same volume of normal saline.The diabetic rat models were duplicated by streptozotocin (STZ). After 4 months of administration, the urinary albumin excretion rates and urinary total protein excretion rates of the rats in various groups were detected. The pathomorphology of the kindey tissue of the rats in various groups were observed by HE staining and the distribution of renal extracellular matrix (ECM) was detected by PAS and Masson staining. The expressions of PTEN protein in kidney tissue of the rats in various groups were detected by immunohistostaining and the expression levels of PTEN protein in kidney tissue of the rats in various groups were detected by Western blotting method. Results:Compared with model group, the glomerular mesangial aggregation, positive staining of PAS as well as Masson aggregation and basement membrane thickening of the rats in HACE group were significantly reduced, and the 24 h urinary albumin excretion rate was significantly decreased (P<0.05),and the urinary total protein excretion rate had no significant difference(P>0.05).The immunohistochemistry staining results showed that the expression level of PTEN protein in kidney tissue of the rats in control group was higher, the expression level of PTEN protein in the kidney tissue of the rats in model group was decreased, and the expression level of PTEN protein in kidney tissue of the rats in HACE group was increased to normal. The Western blotting results showed that the expression level of PTEN protein in the renal cortex tissue tissue of the rats in model group was significantly decreased compared with control group (P<0.05); compared with model group, the expression level of PTEN protein in renal cortex tissue of the rats in HACE group was significantly increased (P<0.05). Conclusion:HACE can increase the expression of PTEN protein in kidney tissue of the rats, which may be one of the mechanisms of its protective effect on the kidney in the diabetic rats.

Key words: herba artemisiae capillaris extracts, diabetes mellitus, diabetic nephropathy, phosphatase and tensin homologue deleted chromatosome 10, extracellular matrix

CLC Number:

R587.2

ZHANG Shuxia, HUO Wenbo, SU Ying, LI Zhen, TIAN Jing, WANG Caixia, SUN Chengbo, ZOU Yinggang, YU Xiaoyan. Effect of herba artemisiae capillaris extracts on PTEN protein expression in kidney tissue of diabetic rats and its protective effect on kidney[J].Journal of Jilin University(Medicine Edition), 2019, 45(04): 779-783.

References

[1] 潘竞锵,刘广南,刘惠纯,等.茵陈蒿对小鼠血糖、血脂的影响[J].中药材,1998, 21(8):408-411.
[2] 潘竞锵,韩超,刘惠纯,等.茵陈蒿汤对正常和多种糖尿病模型动物血糖的影响[J].中药材,2001,24(2):128-131.
[3] 袁效涵,石鹤峰,韩伟峰.加味茵陈蒿汤治疗2型糖尿病40例[J].中医研究,2007,20(2):43-44.
[4] GENG JN,YU XY,LIU CY,et al. Herba artemisiae capillaris extract prevents the development of streptozotocin-induced diabetic nephropathy of rat[J].Evid Based Complement Alternat Med,2018, 2018:5180165.
[5] 孙成博,孙波,刘春禹,等.茵陈提取物对糖尿病大鼠肾组织miRNAs表达谱的影响及其肾脏保护作用[J]. 吉林大学学报:医学版,2018,44(3):493-498.
[6] MAEHAMA T,DIXON J E.The tumor suppressor,PTEN/MMAC1,dephosphorylates the lipid second messenger,phosphatidylinositol 3,4,5-trisphosphate[J].J Biol Chem, 1998, 273(22):13375-13378.
[7] 刘伦华,楼丽广.PTEN功能调节的研究进展[J].中国药理学通报,2005,21(7):778-781.
[8] HADDADI N,LIN Y,TRAVIS G,et al.PTEN/PTENP1:Regulating the regulator of RTK-dependent PI3K/Akt signalling,new targets for cancer therapy[J].Mol Cancer, 2018, 17(1):37.
[9] LEE YJ,HAN HJ.Troglitazone ameliorates high glucose-induced EMT and dysfunction of SGLTs through PI3K/Akt, GSK-3β, Snail1, and β-catenin in renal proximal tubule cells[J].Am J Physiol Renal Physiol,2010,298(5):F1263-F1275.
[10] HAO J,LIU S,ZHAO S,et al.PI3K/Akt pathway mediates high glucose-induced lipogenesis and extracellular matrix accumulation in HKC cells through regulation of SREBP-1 and TGF-β1[J].Histochem Cell Biol,2011,135(2):173-181.
[11] WANG XM,YAO M,LIU SX,et al.Interplay between the Notch and PI3K/Akt pathways in high glucose-induced podocyte apoptosis[J].Am J Physiol Renal Physiol,2014,306(2):F205-F213.
[12] LI X Y,WANG SS,HAN Z,et al.Triptolide restores autophagy to alleviate diabetic renal fibrosis through the miR-141-3p/PTEN/Akt/mTOR pathway[J].Mol Ther Nucleic Acids,2017,9:48-56.
[13] WANG H,FENG Z,XIE J,et al.Podocyte-specific knockin of PTEN protects kidney from hyperglycemia[J].Am J Physiol Renal Physiol,2018,314(6):F1096-F1107.
[14] SUN J,LI ZP,ZHANG RQ,et al.Repression of miR-217 protects against high glucose-induced podocyte injury and insulin resistance by restoring PTEN-mediated autophagy pathway[J].Biochem Biophys Res Commun,2017,483(1):318-324.
[15] 邢玲玲,傅淑霞,杨林,等.PTEN在糖尿病肾病患者足细胞损伤中的作用[J].临床与实验病理学杂志,2014,30(12):1375-1378.
[16] MAHIMAINATHAN L,DAS F,VENKATESAN B,et al. Mesangial cell hypertrophy by high glucose is mediated by downregulation of the tumor suppressor PTEN[J].Diabetes,2006,55(7):2115-2125.
[17] CAMPION CG,SANCHEZ-FERRAS O,BATCHU SN.Potential role of serum and urinary biomarkers in diagnosis and prognosis of diabetic nephropathy[J].Can J Kidney Health Dis,2017,4:2054358117705371.
[18] 王圆圆,刘瑞霞,郭兵,等.大鼠肾组织PETN表达下调在糖尿病肾病发展中的作用[J].生理学报,2011,63(4):325-332.
[19] 程悦,王代红,张耀全,等.PTEN编码蛋白对TGF-β1刺激大鼠肾成纤维细胞分泌Col Ⅳ、FN的抑制作用[J].解放军医学杂志,2007,32(7):719-721.
[20] HLOBILKOVÁ A,KNILLOVÁ J,BÁRTEK J,et al.The mechanism of action of the tumour suppressor gene PTEN[J].Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 2003, 147(1):19-25.

Effect of herba artemisiae capillaris extracts on PTEN protein expression in kidney tissue of diabetic rats and its protective effect on kidney

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