Abstract:

To investigate the association between the single nucleotide polymorphism(SNP) of interferon-gamma(IFN-γ) gene +874 site and susceptibility to ovarian cancer,and provide a theoretical basis for the gene diagnosis of ovarian cancer. Methods Using the PCR,cloning and sequencing techniques,the genotypes of IFN-γ gene +874 site A/T of 87 cases of ovarian cancer patients(case group) and 86 normal women(control group) were detected.Results The distribution of genotypic frequency did not deviate from Hard-Weinberg  equilibrium in both case group and control group;the genotypic frequencies of AA,AT and TT  had no significant difference between case group and control group (P>0.05);the frequencies of IFN-γ gene +874 site T and A allele showed significant difference between case group and control group (P<0.05),the relative risk suffering from T allele was 1.80 times of A allele;the relative risk suffering from TT genotype was 2.99 times of AA genotype;the genotypic frequency and allelic frequency had no significant difference between epithelial ovarian cancer and non-epithelial ovarian cancer in case group (P>0.05).Conclusion T allele of IFN-γ gene+874 site might have a relationship with generation of ovarian cancer,TT genotype might be susceptibility genotype for ovarian cancer.

Key words: ovarian neoplasm;interferon;genotype;single nucleotide polymorphism