Abstract:

To investigate the relationship between the expressions of  P16 and proliferating cell nuclear antigen(PCNA) in osteosarcoma and clinicopathological features,and explore the effects of them in occurrence and development of osteosarcoma.
Methods The expressions of P16 and PCNA were detected by immunohistochemistry(SP) in 71 osteosarcima tissues and 10 normal bone tissues.
Results  ①The positive rate of P16 expression in osteosarcoma tissues was lower than that in normal bone tissues(P<0.05),the positive rate of PCNA expression in osteosarcoma tissues was higher that in normal bone tissues(P<0.05).②The expression of P16 was correlated with osteosarcima’s histological grade and prognosis(P<0.05),and wasn’t correalted with histological type and clinical stage(P>0.05).The expression of PCNA had positive relationship with osteosarcoma’s prognosis(P<0.05),and had no relationship with histological type and clinical stage and histological grade (P>0.05).③There was negative correlation between P16 and PCNA expressions(r^_s=－0.58,P<0.05).
Conclusion P16 and PCNA can play an important role in the occurreace,development and the metastasis of osteosarcoma,and have close relationship with its prognosis.

Key words: osteosarcoma；P16；proliferating cell nuclear antigen；immunohistochemistry