Journal of Jilin University Medicine Edition ›› 2013, Vol. 39 ›› Issue (1): 16-18.doi: 10.7694/jldxyxb20130105

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Induction of tumor necrosis factor related apoptosis-inducing ligand of apoptosis of non-small cell lung carcinoma cells and its mechanism
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ZHENG Zhong-hua1, LI Yun-qian2, GUO Min1, YU Hong-quan2, DING Li-juan2, QI Ling1   

  1. (1.Department of Pathology,Jilin Medical College,Jilin 132013,China;2.Department of Neurosurgery, First Hospital,Jilin University,Changchun 130021,China)
  • Received:2012-06-20 Online:2013-01-28 Published:2013-01-30

Abstract: To study the apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) in non-small cell lung carcinoma H596 cells,and to explore the role and mechanism of apoptosis.Methods Non-small cell lung carcinoma H596 cells were cultivated,and acid phosphate assay was used to detect the apoptotic rate,and the expressions of caspase-8,Bcl-2 and Fas-associated death domain (FADD) proteins were detected by Western blotting in H596 cells.Results  The apoptotic rates of H596 cells were decreased after the cells were treated with 0.01-0.03 μg•L-1 TRAIL,and from 0.10 μg•L-1 TRAIL,the apoptotic rates were increased;the differences in apoptotic rates of H596 cells after treated  with 10.00-100.00 μg•L-1 TRAIL were significant compared with control group(P<0.05).The Western blotting results showed that the expressions of caspase-8,Bcl-2,FADD proteins were normal.Conclusion High dose of TRAIL can induce apoptosis in H596 cells,and it may be  related to the normal expressions of   TRAIL related proteins.

Key words: carcinoma, non-small cell lung, tumor necrosis factor related apoptosis-inducing ligand, apoptosis

CLC Number: 

  • R734.2