Abstract:

Objective
To study the protective effects of the polyphenols of vitis amurensis Rupr(PVAR) on oxidative damage in rats caused  by chronic alcohol intakes.Methods Forty male Wistar rats were divided into 4 groups according rats’ weights: normal control group,alcohol injury group(the rats were treated with 10mL·kg^-1    33% alcohol via i.g);PVAR group(after ingesting 200 and 400 mg·kg^-1   PVAR,the male rats were treated with 10 mL·kg^-1    33% alcohol via i.g).After 8 weeks,the concentrations of malondialdehyde(MDA),reactive oxygen species(ROS),glutathione(GSH) and the activities of SOD and heme oxygenase-1(HO-1) in rat liver tissues were measured.The activities of serum glutamic-pyruvic transaminase(GPT) and glutamic oxaloacetic transaminase(GOT) in rats  were measured.Results Compared with alcohol injury group,the concentrations of MDA,ROS,GPT and GOT were decreased significantly (P<0.05),and the GSH level was increased significantly(P<0.01) in PVAR groups.The activities of SOD,HO-1 in PVAR groups were increased significantly(P<0.05).Conclusion PVAR has protective effects on oxidative damage induced by chronic alcohol intakes and the effects are related to increasing HO-1 activity,scavenging oxygen free radicals and reducing lipid peroxidation.

Key words: polyphenols of vitis amurensis Rupr;chronic alcohol intakes;oxidative damage