Abstract:

Objective To investigate the suppression to  2-glycoprotein-1-specific B cell response  by human recombinant  2-glycoprotein-1 domain Ⅰ dimer（rhβ2-GP1-DⅠ₂） in rhβ2-GP1 immunized mice. Methods The effects of treatment using rhβ2-GP1-DⅠ₂ on titer and ratio of anti-β2-GP1 were  analyzed by solid phase ELISA. The number of splenic β2-GP1-specific antibody-forming cells (AFC) was counted  by  ELISPOT.Results The levels of anti-β2-GP1 from rhβ2-GP1 immunized mice treated with rhβ2-GP1-DⅠ₂  were significantly decreased compared with those in negative controls（P<0.01）.The reductions paralleled by decreases in ratio of  anti-β2-GP1（P<0.01）；β2-GP1-specific AFC decreased at the same time（P<0.01）.
Conclusion Intravenous administration of rhβ2-GP1-DⅠ₂ could inhibit the  2-glycoprotein-1-specific B cell response in rhβ2-GP1 immunized mice.
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Key words: recombinant human , 2-glycoprotein-1； domain Ⅰ dimer；anti-phospholipid antibody syndrome；B lymphocyte tolerogen