Abstract:

Abstract:Objective To explore the effect of collagen  membrane combined with recombinant human bone morphogenetic protein-2(rhBMP-2) on periodontal tissue regeneration  and repair in periodontal  defect models of rats.Methods Eighteen male  healthy Wistar rats,which were made experimental periodontal bone defects in the inferior incisors,were randomly divided into three groups:control group,collagen membrane group (Co),collagen membrane and rhBMP-2 group (Co/rhBMP-2). Two rats from each group
were sacrificed at 4,6,8 weeks after operation. The mandibles were removed,and examined under light microscope.Results In Co/rhBMP-2　 group: 4 weeks after operation, a large amount of bone   formed in the defects;6 weeks later,new bone filled in the defects;8 weeks later,alveolar bone emerged,newly-formed periodontal ligament and cementum localized at the root edges,no gingival epithelium was observed.In Co group:4 weeks after operation, new bone  formed at the edge of the defects;6 weeks later,a larger amount of bone and periost were observed;8 weeks later,a small amount of periodontal ligament and cementum localized at the root edges.In control group:  4 weeks after operation, there was  a small amount of newly-formed bone at the edge of the defects;6 weeks later,collagen fibers were found at  the edge of defects;8 weeks later,a small amount of periodontal tissue reached its original height and gingival epithelium proliferated obviously.
Conclusion Collagen membrane combined with rhBMP-2 which has not only conductive effect but also effect of osteoinduction,can prevent the long-epithelium growing,maintain the growth gap,and promote periodontal tissue to regenerate.

Key words: collagen membrane；recombinant human bone morphogenetic protein-2；guided tissue regeneration