Abstract:

Objective To investigate the association between  methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism and  habitual abortion in   northeast China population,interpret its possible molecular basis,and provide scientific evidence for this disease’s gene diagnosis and provide new ideas of precaution,therapy and prognosis for the women with susceptibility to habitual abortion.
Methods Polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) was used to detect the MTHFR gene C677T polymorphism in 50 normal　 health control women with breed history (control group) and 56 cases with habitual abortion (case group).Results MTHFR gene C677T existed three genotypes:CC,CT,and TT.The genotypic frequency  of CC,CT and TT had  significant difference between control and case groups (χ²=6.254，P=0.044).The genotypic frequency had significant difference between TT and CC (χ²=6.345，P=0.012).The genotypic frequency had no significant difference between TT and CT (χ²=2.099，P=0.147).The allelic frequency had significant differences between T and C (χ²=7.054，P=0.008，OR=2.098,  95%CI:1.210-3.636). Conclusion MTHFR gene C677T polymorphism has relationship with habitual abortion from northeast China population and T allele may be one of the genetic susceptibility factors to habitual abortion.

Key words: habitual abortion;methylenetetrahydrofolate reductase;C677T polymorphism