Abstract:

Abstract:Objective To observe the protective effects of ATP and panax ginseng triol set on the denerved muscle atrophy and motor end plate,and provide basis for the selection of operation opportunity in patients with never injury.Methods Adult male Wistar rats were used. The model of denervated gastrocnemius muscle was established by cutting off the sciatic nerve.The rats were randomly divided into control group,ATP group,low dose panax ginseng triol set (25 mg·kg^-1) group and high dose panax ginseng triol set (50 mg·kg^-1) group. During four weeks after operation, ATP was injected into gastrocnemius and the panax ginseng triol set was injected into belly. The muscle wet weight，diameter of myocytes，cross section area of myocytes and the morphological changes of motor end plate were measured 2,4,6,8,12,16,20, and 24 weeks after operation.  Results  After nerve injury, the muscle wet weight，diameter of myocytes，cross section area of myocytes were decreased. During 16 weeks after nerve injury, there were significant differences between ATP group , low dose panax ginseng triol set group, high dose panax ginseng triol set group and  control group(P＜0.01). At 20 weeks after nerve injury, there was no difference between ATP group and control group, but there were differences between low dose panax ginseng triol set group, high dose panax ginseng triol set group and  control group(P＜0.05). At 24 weeks after nerve injury, there were  no differences between  four groups. There were no significant changes of motor end plate during  4 weeks after nerve injury. Four weeks later, the motor end plate lose its normal morphous and changed into neurofilaments. Until 16 weeks after operation，the neurofilaments began to decrease. The number of neurofilaments in ATP and panax ginseng triol set groups was more than that in control group.  Conclusion  The denerved muscle atrophy and motor end plate cataplasia could be deferred by using ATP and panax ginseng triol set.

Key words: peripheral nerve, nerve injury, amyotrophy, panax ginseng triol set