Abstract:

Abstract:Objective
To investigate the effect of the ultra-filtration extract mixture from Angelica Sinensis and Hedysarum Polybotrys on heat shock protein 70(Hsp70)expression in cardiomyocytes,and clarify its antioxidation on cardiomyocytes．Methods Myocardial cells from 1-3 d neonatal rats were cultivated in DF medium and the cellular injury was induced by H₂O₂（400 μmol•L^-1）．The ultra-filtration extract mixtures were given in three doses of 3.75，7.50， and 15.00  g•L^-1．The beating rates of cardiomyocytes were observed by phase-contrast microscope.The apoptotic rates of cardiomyocytes were measured by flow cytometry．The expressions of Hsp70 mRNA and protein in cardiomyocytes were measured by RT-PCR and Western blotting．Results Compared with normal control group,the beating rates of cardiomyocytes were significantly decreased (P<0.01) and the apoptotic rates of cardiomyocytes were significantly increased(P<0.01) in model group,and the expressions of Hsp70 mRNA and protein in cardiomyocytes were increased(P<0.05，P<0.01).Compared with model group,the beating rates of cardiomyocytes were significantly increased(P<0.01) and the apoptotic rates were significantly decreased (P<0.01) in each treatment group,and the expressions of Hsp70 mRNA and protein in cardiomyocytes were significantly increased（P<0.01，P<0.05）.Conclusion The ultra-filtration extract mixture has the protective effects on cardiomyocytes injured by H₂O₂ and can up-regulate the Hsp70 expression in cardiomyocytes．

Key words: ultra-filtration extract mixture from Angelica Sinensis and Hedysarum Polybotrys；apoptosis；myocytes,cardiac；HSP70 heat-shock proteins