Abstract:

Abstract:Objective To label anti- c-erbB-2 monoclonal antibody  with ¹³¹I,and investigate its inhibitory effect on the growth of ovarian cancer in mude mice.Methods Anti-c-erbB2 monoclonal antibody was combined with ¹³¹I to produce radioimmunological targeting drug.The nude mice which had been inoculated subcutaneously with ovarian cancer SKOV3 cells and the diameter of tumor mass was about 1.0 cm were randomly divided into 3 groups:G₁,G₂ and control groups.The mice in G₁　and G₂ groups were treated with 11.1　MBq and 3.7　MBq anti-c-erbB2 monoclonal antibody labeled with ¹³¹Irespectively.The mice in control group was treated with mIgG labeled with ¹³¹I.The size and weight of tumor were determined once every week during 6 weeks after administration.Results Compared with  control group, the  size and weight of tumor in G₁ and G₂ groups were significantly decreased,and the inhibitory rate was increased(P<0.05). The size and weight of tumor in G₁　group were lower than those in G2 group(P<0.05),and the inhibirory rate in G1 group  was higher than that in G₂ group(P<0.05) .Conclusion Anti-c-erbB-2 monoclonal antibody labeled with radionuclide ¹³¹Icould inhibit the growth of ovarian cancer in nude mice and the effect of 11.1 MBq c-erbB-2 monoclonal antibody labeled with ¹³¹Iis better than 3.7 MBq  ¹³¹I-c-erbB-2.

Key words: ovarian neoplasms;c-erbB-2;antibodies monoelonal;¹³¹I; , radioimmunotherapy