Abstract:

Abstract:Objective To construct the dual-subtype (H5/H7) influenza virus DNA vaccine and determine its immunogenicity.Methods Influenza virus DNA vaccine pVAX1-H5HA-H7HA co-expressing H5 subtype hemagglutinin (HA) and H7 subtype HA was constructed.The expressed target proteins in BHK cells were identified by IFA.Immunized mice were divided into four groups(15 mice in each group):pVAX1-H5-H7,pVAX1-H5,pVAX1-H7 and pVAX1 control groups,and the serum antibodies were detected by ELISA,the number of splenic T lymphocyte subgroups was determined by flow cytometry.Results The product of constructed dual-subtype (H5/H7) influenza virus DNA vaccine in BHK cells stimulated the expression of green immunofluorescence of fluorescent antibody,and the pVAX1 empty vector transfected BHK cells had no immunofluorescence.The immunized mice produced the serum specific antibodies.The number of splenic T lymphocyte subgroups CD4+ and CD8+ was  higher than that in control group（P<0.05）.Conclusion Dual-subtype (H5/H7) influenza virus DNA vaccine is successfully constructed and this DNA vaccine can induce specific cellular and humoral immune responses in mice.

Key words: influenza A virus;vaccines,DNA;humoral immunity;cellular immunity