Abstract:

Abstract:Objective To observe the effects of  20(s)-protopanaxadiol (PPD) on the growth of prostate cancer cells RM-1 and explore the mechanism of its action.Methods The mouse models with prostate cancer were constructed with RM-1 cells.36 mice C57 were randomly divided into three groups:model group(fed with  an equal volume of olive oil once a day,an equal volume of normal saline injection  intraperitoneal  once a week),paclitaxel(Taxol) group(20 mg/kg,intraperitoneal injection  once a week),PPD group(20 mg/kg,ig once a day),continuous administration of 4 weeks.The tumor size was measured once every 3 d,and the morphological changes of tumor were observed,the animals were killed over the fourth weekend.Observation:the general state of animal tumor weight,tumor volume,inhibitory rate,mortality;and the expression of Cyclin D1 gene was detected with RT-PCR and Western blotting.Results Compared with model group,the tumor growth of mice in other groups significantly slowed down,and the mice in PPD group had bright hair.The mice in  model group  scrambled and moved slowly with tumor ulceration,erosion and hemorrhage.The tumor volume of the mice in PPD group was significantly smaller than that in model group(P<0.05) 13 d after administration,and it was also significantly smaller than that in Taxol group(P<0.05) 21 d after administration.Compared with model group,the tumor weight of the mice in PPD group was significantly decreased(P<0.05),but the inhibitory rate was significantly increased(P<0.05),no death.Compared with Taxol group,the inhibitory rate in PPD group was significantly increased(P<0.05),the mortality was significantly decreased(P<0.05).The RT-PCR and Western blotting results showed that Cyclin D1 gene and protein expressions in treatment  groups were significantly reduced(P<0.05)compared with model group;compared with Taxol group,the expression of Cyclin D1 in PPD group was significantly decreased(P<0.05),but there was no significant difference of Cyclin D1 protein expression between PPD group and Taxol group(P>0.05).Conclusion PPD can significantly inhibit the growth of prostate cancer cells RM-1 and its mechanism may be related to the down-regulation of the expression of Cyclin D1.

Key words: 20(s)-protopanaxadiol;prostate neoplasms;Cyclin D1