Abstract:

Abstract:Objective To study the effect of low dose radiation(LDR) on the kidney function and morphology  in C57BL/6J mice with  diabetic nephropathy(DN)induced by streptozotocin(STZ) and illuminate the protective function of LDR on kidney damage caused by diabetes mellitus(DM).Methods The healthy and right age C57BL/6J mice were divided into 4 groups including control,DM,LDR and DM/LDR.The mice in DM and DM/LDR groups were injected intraperitoneally with STZ to set up DM models.The mice in DM/LDR and LDR groups were irradiated with 25 mGy X-rays every other day for 4 weeks.The changes of blood glucose level,urine index level and the morphology of glomerular were detected at 2,4,8,12,16 weeks after radiation.Results The blood glucose levels of mice in DM and DM/LDR groups after STZ-induced DM model preparation were higher than those in LDR and control gourps(P<0.05).After treated with LDR for 2 weeks,the blood glucose level in DM/LDR group was supressed and significantly lower than that in DM group (P<0.05).Moreover the the change  had been kept to 16 weeks.  In addition,compared with DM group,the level of urine micro albumin(MALB) in DM/LDR group was decreased and the urine creatinine(Cre) level was increased.Compared with DM group,the morphological results showed that the glomerular mesangial expansion and mesangial cell proliferation were significantly supressed in DM/LDR group(P<0.05).Conclusion LDR can promote the decease of blood glucose level efficiently,relief the change of kidney fuction,supress and delay the pathological changes of DN.

Key words: low dose radiation;renal function;creatinine;micro albumin;morphology detection