Abstract:

Abstract:Objective  To construct the acute myocardial infarction models in rats with blood stasis and study the difference on blood biochemics between the acute myocardial infarction models  with blood stasis and the simple acute myocardial infarction models.Methods Wistar rats were randomly divded into   control group, acute blood stasis model group,acute myocardial infarction sham operation group, acute myocardial infarction model group and of acute myocardial infarction model with blood stasis group.The acute myocardial infarction models under the status of the acute blood stasis in rats were set up.The serum malondialdehyde (MDA),nitric oxide(NO),free fatty acid (FFA),tumor necrosis factor-α (TNF-α) levels were detected,the activities of serum superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and the levels of prostacycline(PGI2),thromboxane A2 (TXA2) and endothelin (ET) in plasma were determined.Results There were not obvious differences in MDA,SOD,GSH-Px and FFA between the acute myocardial infarction models with blood stasis in rats and the simple acute myocardial infarction models(P<0.05).The decrease levels of PGI2 and NO,and the increase extents of TXA2,ET and TNF-α in the acute myocardial infarction models in rats with blood stasis were  higher than those in the simple acute myocardial infarction models(P<0.05).Conclusion The changes of the myocardial damage induced by oxygen free radical and the metabolic disorder of FFA resulted from the myocardial ischemia are not significant,while the releasing contents of interior active mature,such as ET,FFA,TXA2 and NO,are significant when the acute myocardial infarction models in rats with blood stasis and the simple acute myocardial infarction models are compared.The results show that it is  defective to evaluate pharmacodynamics of traditional Chinese drug with only simple acute myocardial infarction models.

Key words: acute blood stasis, acute myocardial infarction, disease model,animal;free , radicals;vasoactive factors