Abstract:

bstract:Objective
To obain the C terminal gene encoding human mannose-binding lectin associated serine protein kinase-2(MASP-2),and express human MASP-2 C-terminal fragment and clinical application in E.coli and lay foundation for preparation of monoclonal antibodies and clinical application in relevant diseases. Methods The cDNA of human MASP-2 C-terminal was amplified from total RNA extracted from human fetal liver tissue with RT-PCR,and was cloned into pGEX-6p-2 vector and identified by restrictive digestion analysis and sequencing. Results The cDNA encoding human MASP-2 C-terminal was isolated,linked with pGEX-6p-2 vector and transformed into E.coli XL1-blue. The restriction map analysis and sequencing results were consistent with the sequence of MASP-2 C-terminal cDNA that  reported in GenBank. Conclusion The human MASP-2 C-terminal cDNA is successfully cloned and expresses in E.coli.

Key words:  mannose-binding lectin associated serine protein kinase-2 C;reverse transcriptase polymerase chain reaction;cloning,molecular;gene expression