Abstract:

Abstract:Objective To investigate the effect of low dose irradiation on the expression changes  of Cyt c and caspase-3 in mouse testis,and  interpret the effects  on  apoptosis in mouse testis induced by irradiation. Methods The mice were divided into different doses（0,0.050,0.075,0.100and 0.200 Gy）groups and different time course (0,6,12 and 24 h) groups. The expressions of Cyt c and caspase-3 mRNA and proteins in mouse testis tissues irradiated with different doses X-rays were observed by real time PCR and Western blotting.Results The real time PCR results indicated that the expressions of Cyt c and caspase-3mRNA were increased with the dose increasing 12 h after irradiation with different doses of X-rays,and the expression of Cyt c mRNA reached the peak after irradiated with 0.100 Gy,but the expression of caspase-3 mRNA expression reached the peak after irradiated with 0.075 Gy;the expression levels of Cyt c and caspase-3protein were the highest when irradiated with 0.075 Gy X-rays. The expressions of Ct c and caspase-3 mRNA after irradiation with 0.075 Gy X-rays were increased with the time prolongation,and reached the peak after 24 h,but the Cyt c and caspase-3 proteins expreesed  the most 12 h after irradiation with 0.075 GyX-rays. Conclusion The mRNA and protein expressions of Cyt c and caspase-3 in mouse testis spermatogenic cells can be increased by low dose irradiation with dose- and time-effect regularity.

Key words: Cyt c;caspase-3；real time PCR;irradiation;spermatogenic cell;mice