Abstract:

Abstract:Objective To  study the potential for multiple differentiation of human umbilical cord mesenchymal stem cells (UCMSCs) and discuss the repair effects of UCMSCs in treatment of hypoxic-ischemic brain damage(HIBD) in rats. Methods The UCMSCs were isolated and cultured by using tissue digestion and adherent culture;the expressions of mesenchymal stem cells(MSCs) specific markers of  the P4 and P10 UCMSCs were detected by  flow cytometry,and the aberration condition was judged by chromosomal tests;the potential of multiple differentiation was detected by neurogenic,adipogenic and osteogenic lineages. The active avoidance response rate (AARR) and none avoidance response rate (NARR) were detected by shuttle box test in  normal control,abdominal transplantation UCMSCs and HIBD groups. Results The flow cytometry results showed strong expressions of CD29,CD44 and CD105,and very low expressions of CD34 and CD45 in these.The  immunofluorescence and cell staining results showed that the UCMSCs could be induced into neural cells,adipocytes and osteoblasts;even after high passages.The UCMSCs could still be  differentiated into multiple cells and chromosome aberration did not occur. The shuttle box test results suggested that the AARR in UCMSCs transplantation group was high  than that in HIBD  group on the 3th,4th and 5th days (P<0.05). Conclusion The UCMSCs are easy to be confirmed and purified,and they have multiple differentiation potential,which are still stable with high passages.

Key words: human umbilical cord mesenchymal stem cells;multi-directional differentiation;hypoxic-ischemic brain damage;chromosome examination;shuttle box