J4 ›› 2011, Vol. 37 ›› Issue (2): 220-225.
Previous Articles Next Articles
SHAN Yan-hong 1,2,ZHANG Hai-jun2,XU Zhi-gang*,CUI Man-hua1
Received:
Online:
Published:
Abstract:
Abstract:Objective To observe the inhibitory effects of curcumin(Cur) associated with cytokine-induced killer (CIK) cells on proliferation of ovarian carcinoma SKOV-3 cells in vitro,and discuss whether they have synergetic anti-tumor effects ifthey are used together and the possible mechanism. Methods The CIK cells were induced from umbilicus cord blood,and the SKOV-3 cells were randomly divided into Cur group,CIK cells group and Cur associated with CIK cells group.The inhibitory rates of proliferation in each group were detected by MTT method. The mRNA levels of Fas,Fas associated death domain protein(FADD) and Caspase-3 in each group were measured by RT-PCR. Results Compared with Cur and CIK cells groups,the inhibitory rate of proliferation of SKOV-3 cells in Cur associated with CKI cells group was increased,and the inhibitory rate was increased with the time prolongation and dose increasing. The inhibitory rate of SKOV-3 cells approached 76.2% when the concentration of effector to taet ratio was 12.5∶1 and 20 μmol·L-1 Cur was used for 〖JP2〗48 h.The mRNA 〖JP3〗levels ofFas and Caspase-3 〖JP〗in SKOV-3 cells were increased in Cur associated with CIK cells group compared with Cur and CIK cells groups.The mRNA levels of FADD didn’t have significant changes in various groups.Conclusion The effect of anti-tumor can be enhanced in a synergetic pattern when Cur and CIK cells are used together. Its mechanism may be related with the promoting effects on the mRNA levels of Fas and Caspase-3 in SKOV-3 cells when they are used together.
Key words: curcumin, cytokine-induced killer cells, ovarian carcinoma cells;cell proliferation;apoptosis
CLC Number:
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://xuebao.jlu.edu.cn/yxb/EN/
http://xuebao.jlu.edu.cn/yxb/EN/Y2011/V37/I2/220
Cited