Abstract:

Abstract:Objective To construct three truncated SCIRR 10 protein mammalian cells systems,and discuss the protein function sites of SCIRR 10. Methods Three truncated SCIRR 10 genes were amplified by PCR,and three kinds of recombinant eukaryotic expression vectors were constructed as follows:pcDNA3.1/Myc-His-SCIRR 10(1-145aa)，pcDNA3.1/ Myc-His- SCIRR 10(1-90aa) and  pcDNA3.1/Myc-His-SCIRR 10(1-70aa). Then they were transiently transfercted intoCOS-7 cells at the same time. After 48 h,the recombinant proteins were detected by Western blotting. Results The molecular weights of  three truncated SCIRR 10 proteins  were    18 800,12 800 and  10 700.Three SCIRR 10 truncated proteins were  successfully expressed in COS-7 cells.β-tubulin protein had no change. Conclusion The three truncated SCIRR 10 protein mammalian cell systems are constructed successfully,and the proteins are obtained by this system.

Key words: spinal cord injury and regeneration related gene No10;truncation;construction;protein expression