Abstract:

Abstract:Objective To investigate the reversal effect of ganoderma lucidum polysaccharides (GLPS) on drug resistance in ovariancancer cells SKOV-3/DDP  and clarifyits drug-resistance  mechanism of GLPS through  improving the resistance genes of ovarian cancer. Methods The ovarian cancer cells SKOV-3/DDP were treated with  different concentration gradients of GLPS (4 ,40 and 400 mg·L^-1) and cisplatin (1,10 and 100 mg·L^-1), the inhibitory rates were detected by MTT method  per 12 h (total test 12-72 h),the experimental cells were divided into control group,GLPS group and cisplatin groupwhen the best inhibitory concentration was gotten. The expressions of  related resistantce genes GSTM1,GSTT1 and GSTP1 were detected by  real-time PCR. The expression differences of GSTM1-related   resistance protein were detected by Western blotting method. Results The inhibitory rates of SKOV-3/DDP cells were higher after treated with  40 and 400 mg·L^-1  GLPS for 12-72 h,and the inhibitory rate was increased with the time prologation and the increase of concentration (P<0.05),and the best inhibitory concentration was 40  mg·L^-1. 10  and 100  mg·L^-1  cisplatin didn’t have effect on the inhibitory rate of SKOV-3/DDP  cells(P>0.05). The expressons of GSTM1,GSTT1 and GSTP1 of OV-3/DDP cells were decreased after treated with 40 mg·L^-1  GLPS  and 10 mg·L^-1  cisplatin for  36 h(P<0.05). The expression of GSTM1 protein was  decreased the most. Conclusion GLPS can inhibit the expressions of drug resistance genes GSTM1,GSTT1 and GSTP1,and improve the resistance effect of tumor cells and enhance their sensitivityies to chemotherapeutic drugs.

Key words: SKOV-3/DDP；ganoderma lucidum polysaccharides；drug resistance genes