Abstract:

Abstract:Objective To establish quantifiable primary and experimental lung metastasis  mouse models by using GFP labeled MDA-MB-231 cell lines and provide basis for the study on metastasis mechanism of breast cancer.  Methods The MDA-MB-231 cells were infected by GFP labeling lentiviral vector-mediated packaging system and inoculated subcutaneously into Balb/C nude mice or through tail vain into SCID mice to set up primary and experimental lung metastasis models.Then the mice were executed at 8 th and 5 th weeks,and the  lungs were  extracted and analyzed by stereomicroscope.  Results 8 weeks after the cells were inoculated in primary lung metastasis mice,the tumor in situ was observed with the diameter of 15 mm. The metastasis foci in the lung were invisible to naked eye,but were visible at 488 nm excitation wavelength with green fluorescence. 5 weeks after the cells were injected in experimental lung metastasis mice,the metastasis foci were still invisible to naked eye,but were clearly visible at 488 nm excitation wavelength with green fluorescence,which were easy to be observed and calculated.  Conclusion The mouse model for lung metastasis using MDA-MB-231 cells with countable green fluorescence foci is successfully established.

Key words: breast neoplasm;lentivirus system;lung metastasis;animal model