Abstract:

Abstract:Objective To observe the inhibitory effect of gemcitabine (GEM) combined with the dendritic cells (DC) sensitized byHepG2 cells’ whole lysates on proliferation of HepG2 cells and provide experimental basis for its clinical application.Methods Whole cell lysates of HepG2 cells were extracted with freeze thawing method,then the monocyte-derived DC were plusedinto this cellular antigen. The HepG2 cells were divided into negative group(only HepG2 cells);unactivated T lymphocytes(UTL) group;activated T lymphocytes(ATLgroup ;50, 100,150,and 200 μg/L^-1  GEM groups;UTL+ 50,100,150,and200 μg/L^-1 GEM groups;ATL+50,100,150,and 200 μg/L^-1  GEM groups.The capability of antigen plused DC to promote T lymphocyte proliferation and the abilities of  different concentrations of  GEM combined with sensitized T cells to kill HepG2 cells were detected by MTT methods,respectively. The apoptotic rates of HepG2cells in various groups were detected by flow cytometry. Results There was significant difference of the stimulate index(SI) between control and negative control groups（P<0.01）.The kill rate in ATL group(21.68%±4.39%) was higher than thatin UTL group （4.81%2.54%）,there was significant difference（P<0.01）.Compared with ATL+50,100,150 μg/L^-1  GEgroups,the kill rate in ATL+ 200 μg?L-1  GEM group was significantly increased（P<0.05）.Compared with negative control group（4.47%±1.98%）,the apoptotic rate in UTL group （5.45%±0.94%)had no significant differenc（P>0.05） ;the apoptotic rate in ATL group （14.32%±1.16%）had  significant differences compared with the former two groups（P<0.01）. The apoptotic rate in ATL+ 100 μg/L^-1  GEM group was 24.52%±0.85%,there were significant differences compared with the other groups（P<0.05）. Conclusion The DC sensitized by HepG2 cells’ whole lysates could induce anti-cancer cytotoxicity T cells and the activated T cells combined with GEM can significantly increase their effects to kill tumor cells .

Key words: primary liver carcinoma;dendritic cell;gemcitabine