Abstract:

Abstract:Objective To observe the effect of creatine phosphate sodium(CP) on the invasion of human ovarian carcinoma SKOV3 cells in vitro and explore its mechanism. Methods The SKOV3 cells cultivated in vitro were treated with normal saline(control group),1,6 and 12 mmol/L^-1CP for 72 h,respectively. The adhesive ability of cells in each group was detected by MTT. The invasive and migrative abilities of cells in each group were detected by Transwell method. The expressions of nm23-h1,c-myc,MMP-2 and MMP-9  were analyzed with RT-PCR and FCM. Results Compared with control group,the indicators mentioned above in 1 mmol/L^-1 CP grooup had no significantly changes (P>0.05);and the invasive and migrative abilities in 6 and 12 mmol/L^-1CP groups were significantly decreased(P<0.05). The RT-PCR and FCM results showed that the nm23-h1 gene and protein expressions in 6 and 12 mmol/L^-1CP groups were significantly increased compared with control group (P<0.05);but there was no significant differencebetween 6 mmol?L-1CP group and 12 mmol/L^-1 CP group (P>0.05). Compared with control group,the expressions of c-myc in 6 and 12 mmol/L^-1 CP groups were significantly decreased (P<0.05),but there was no significant difference between 6 mmol/L^-1CP group and 12 mmol/L^-1CP group (P>0.05). The MMP-2 and MMP-9 protein expressions in 1,6 and 12 mmol/L^-1CP groups had no significant changes compared with control group (P<0. 05). Conclusion CP can significantly inhibit the adhesion,invasion,migration of SKOV3 cells and its mechanism may be related to  up-regulation of the expression of nm23-h1 and down-regulation of the expression of c-myc.

Key words: adenosine analogues;invasion;ovarian neoplasms;nm23 nucloside diphosphate kinases;genes,myc