Abstract:

Abstract:Objective  To study the antagonism of zinc sulfate on damage effect of ultraviolet  B (UVB) on HaCaT cells,and to provide theoretical basis for development of drug to prevent UVB damage. Methods The HaCaT cells were cultivated in RPMI 1640 and randomly divided into control group,Zn group,UVB group and Zn+UVB group.Nothing was administrated to the  cells in control group,the cells in UVB group  were radiated by UVB,the cells in Zn group  were administrated with 50 μmol·L^-1 ZnSO4 and the  cells in  Zn+UVB group were administrated with 50 μmol·L^-1 ZnSO4 24 h before UVB radiation.The  UVB radiation time was 6 min,the  cells in every group were collected 24 h after UVB radiation.The cell viability was measured by trypan blue exclusion assay,the  MDA level was measured by TBA method,the DNA damage in HaCaT cells was me
asured by single cell gel electrophoresis(SCGE).Results  Compared with control group,24 h after radiation,the cell viabilities in UVB group and Zn+UVB group were significantly decreased (P<0.05),the MDA levels were increased (P<0.05).Compared with UVB group,the cell viability in Zn+UVB group was significantly increased (P<0.05),the MDA level was decreased (P<0.05).The SCGE results revealed that  the DNA fragmentation(comet) of the cells in UVB group and Zn+UVB group were induced by UVB. The tail extent moment and Olive tail moment of the cells in UVB group were significantly higher than those in Zn+UVB group (P<0.05).
Conclusion UVB can lead the decreasing of the viability of HaCaT cells,the increasing of MDA level and DNA damage,while zinc sulfuric can antagonize the damage effect of UVB on HaCaT cells.

Key words: ultraviolet , B;HaCaT cells;DNA damage;single cell gel electrophoresis;malonaldehyde