Abstract:

Objective  To construct oxygen-glucose deprivation (OGD) model of human Hela cell line and to investigate the effect of OGD on the apoptosis of tumor cells and its mechanism  in vitro.Methods The OGD models were constructed by Earle’s balanced salt solution (EBSS) and sodium dithionite.Four groups were established such as control group,OGD 4 h group,OGD 8 h group and OGD 12 h group.The changes of proliferation rates of cells in different groups were detected by MTT.The expressions of proteins related to intra-celluar endoplasmic reticulum stress and apoptosis of Hela cells in each group were detected by Western blotting and the morphological changes of nuclear in each group were detected by Hoechst staining.Results Compared with control group,the proliferation rate in OGD group was decreased obviously,the difference had statistical significance（P<0.05）;the expression of GRP78 protein related with endoplasmic reticulum stress was significantly in creased,and the expressions of apoptosis signal protein CHOP and the caspase-4 activated fragment protein were also increased（P<0.05）,while the expression of protein of caspase-3 activated fragment related to intra-celluar apoptosis was also increased（P<0.05).The morphological changes of nuclear appeared in OGD group,such as nuclear crimple and karyorrhexis.Conclusion OGD can induce the apoptosis of human Hela cell line and the apoptosis may be mediated by endoplasmic reticulum stress.

Key words: oxygen-glucose deprivation;endopla