Abstract:

Objective To explore the effect of   autologous hair keratin(HK) composite particles on fibroblasts(HK)  in vitro.
Methods The experiment was divided  into Fbs group,Fbs∶HK=1∶1 group,Fbs∶HK=2∶1 group and HK group.The digestion method was used in primary culture of Fbs;MTT method was used to determine the growth curve of Fbs; the hydroxyproline(HYP) content in   composite culture of Fbs and autologous HK was determined with HYP kit.Results The human  skin Fbs grew well.When the ratio of Fbs to HK  was 2∶1,the HYP content was the highest  in composite culture.In MTT assay,when the radio of Fbs to HK was 2∶1,the grafting efficiency was  perfect.Conclusion Composition of  cultured Fbs in vitro with HK can significantly promote the fragmentation and proliferation of cells;when the ratio of Fbs to HK is 2∶1,it is benifit to promote the synthesis of Fbs into collagen.

Key words: hair keratin;fibroblast;hydroxy