Abstract:

Abstract:Objective To investigate the genetic association between the polymorphisms of estrogen receptor α(ERα) gene and type 2 diabetes mellitus(T2DM)  in the  Han population from North China，and  to discuss the association between single nucleotide polymorphisms (SNPs) of ERα gene and T2DM.Methods 128 DM patients were selected as case group and 193 nomal persons as control group.The  genotypic  frequencies and the  allelic frequencies of rs　11155816,rs 2077647 and rs 9397463 sites in two grops  were compared by  polymerase chain reaction (PCR) technique and  restriction fragment length polymorphism(RFLP) method,the SNPs of rs11155816,rs2077647 and rs9397463 sites of ERα  gene were analyzed, and the relative risk of the haplotypes combined by the three sites mentioned above was analyzed by link analysis of multiple sites.Results The genotypic frequencies of rs11155816,rs2077647 and rs9397463 site of  ERα gene did not deviate from Hardy-Weinberg equilibrium in both case and control groups（P>0.05）.There were no significant differences of the frequencies of the genotypes and the alleles of rs11155816,rs2077647 and rs9397463 sites between two groups（P>0.05）. There were no significant differences among  the haplotypes formated by every two of  rs 11155816,rs 2077647 and rs 9397463 sites（P>0.05）.Conclusion There may be no genetic associations between the polymorphisms of rs11155816,rs2077647 and rs9397463 on ERα gene and T2DM in the Han population from North China.

Key words: diabetes mellitus,type 2;estrogen  , receptor alpha;single nucleotide polymorphismsrs;rs11155816;rs2077647;rs9397463