J4 ›› 2012, Vol. 38 ›› Issue (1): 37-41.
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XU Song-bai1,LIU Bao-bin1,ZHAO Hong-guang2,YU Hong-quan1,LI Yun-qian1,ZHANG Xian-feng1,ZHANG Yu,JIN Zheng1|ZHAO Gang1
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Abstract:Objective To explore the effects of double interfering vector pGenesil-2.1-PTTG-survivin siRNA on PTTG and survivin gene expressions in human U251 glioma cell line. Methods U251 cells were divided in to five groups: normal control,negative control (Lipo2000+ pGenesil-2.1 HK),Lipo2000+pGenesil-2.1-PTTG siRNA,Lipo2000+ pGenesil-2.1- survivin siRNA and Lipo2000+pGenesil-2.1-PTTG-survivin siRNA groups.The PTTG and survivin protein and mRNA levels in every group were measured with flow cytometry,Western blotting and RT-PCR method after U251 cells were transfected with these plasmids. Results The PTTG and survivin protein and mRNA levels in Lipo2000+pGenesil-2.1 PTTG siRNA,Lipo2000+ pGenesil-2.1 survivin siRNA and Lipo2000+pGenesil-2.1-PTTG-survivin siRNA groups were decreased significantly compared with negative control group(P<0.01),especially in pGenesil-2.1-PTTG-survivin siRNA group. Conclusion The silence effects of double interfering vector pGenesil-2.1-PTTG-survivin siRNA on PTTG and survivin gene in U251 cells are stronger than those of pGenesil-2.1-PTTG siRNA or pGenesil-2.1- survivin siRNA.
Key words: pituitary tumor transforming gene;survivin;RNAi;glioma
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XU Song-bai,LIU Bao-bin,ZHAO Hong-guang,YU Hong-quan,LI Yun-qian,ZHANG Xian-fen. Silence effects of double interfering vector targeting PTTG and survivin genes on U251 cells[J].J4, 2012, 38(1): 37-41.
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http://xuebao.jlu.edu.cn/yxb/EN/Y2012/V38/I1/37
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