Abstract:

Objective To study the anticoagulant activities of polysaccharide and its sulfated polysaccharide from corn-cob and mechanism,and lay foundation for their clinical applications. Methods The water-soluble crude polysaccharide(ACC) was extracted by acid from corn-cod and classification,purification and sulfation were performed, physiological saline was used as control group,the ACC,purifid polysaccharide(ACP) and sulfated polysaccharide(ACS) were divided intothree groups according to low,medium and high concentrations. The bleeding time(BT) and clotting time(CT) of small white mice and the activated partial thromboplastin time (APTT)，thrombin time (TT) and proth rombin time (TT) of mix-plasma from the human were detected. Results
The ACC extracted from 1 kg corn-cod  was 12.5 g;the total sugar content was 59%，G.C. analysis indicated that it contained glucose〖JP3〗 (Glc),xylose (Xyl) and galactose (Gal),and its molar ratio was 15.36∶2.67∶1.00， The total purifid sugar of the ACP was 90.2%， G.C. analysis indicated that it contained glucose (Glc),xylose (Xyl) and galactose (Gal),and its molar ratio was 3.90∶2.12∶1.00;Ion chromatographic analysis showed that the  sulfate content of ACS was 9.86%.Compared with control group,except low and medium concentrations  groups,the  CT and BT in the other groups were  prolonged (P<0.05 or P<0.01),the APTT and TT  in human  mixed plasma were significantly prolonged (P<0.05 or P<0.01) in  concentration-dependent manner,PT had  no change. Conclusion ACC has anticoagulant activity  and perfrom its anticoagulant activity  by endogenous pathway of blood coagulation and anticoagulant effect of common pathway of blood coagulation.

Key words: corncob;polysaccharide;sulphated polysaccharide;anticoagulation