Abstract:

Objective To investigate the influence of juglon on adhesion，activities and expressions of  matrix  metalloproteinases（MMP2 and MMP9） in human colon carcinoma HCT-8 cells,and clarify the possible mechanism that juglone can inhibit the invation and migration of tumor. Methods The human colon carcinoma HCT-8cells cultivated in vitro at logarithmic growth phase were divided into 1.25,2.50,5.00,10.00 and  20.00 mg.L^-1 juglone groups,while the control group was set up. The ability of cell adhesion was detected by fibronectin adhesion assay after treated for 72 h，the activities of MMP2 and MMP9 of the cells were detected by gelatin zymography analysis，the expressions of MMP2 and MMP9 proteins were examined by Western blo
tting method. Results Compared with control group, the adhesion rates of HCT-8 in 1.25，2.50，5.00，10.00 and 20.00 mg.L^-1juglone groups were decreased（P<0.05);the activities of MMP2 in 2.50，5.00，10.00 and 20.00 mg.L^-1 juglone groups was decreased;the activities of MMP9  in all juglone groups with different concentrations were decreased（P<0.05）;the expressions of MMP2 and MMP9 proteins in juglone groups with different concentrations were decreased（P<0.01）. Conclusion Juglone could significantly inhibit the adhesion ability of HCT-8 cells and decrease the activities and protein expressions of MMP2 and MMP9. It is suggested that the
decreasing of the activities and protein expressions of MMP2 and MMP9  might be the possible mechanism that juglone can inhibit the  adhesion  of  HCT-8 cells in vitro.

Key words: juglone；cell adhesion；matrix , metalloproteinases;colon neoplasms