Abstract:

Objective
To detect the hMOF protein expression in renal cell carcinoma(RCC) tissue,and explore its relationship with the cliniopathological characteristics of kidney carcinoma.Methods Immunohistochemistry and qRT-PCR were used  to detect the hMOF expression in the normal kidney tissues and renal cell carcinoma tissues.The relationship between the hMOF expression and clinical features of kidney carcinoma  was analyzed.Results The　 qRT-PCR results showed that there were 4 cases in 9 cases of RCC (44.4%) which hMOF mRNA levels were decreased significantly.The immunohistochemistry results showed that there were 39 cases (62.1%,39/66) of RCC  which hMOF protein expression was negative or weakly positive,only 4 cases (6.1%,4/66) of normal kidney tissues which hMOF protein expression was negative or weakly positive,there was significant difference between two groups (P<0.05). The hMOF expression  in Fuhrman Ⅲ kidney carcinoma tissues  was significantly lower than that in Fuhrman Ⅰ-Ⅱ(P<0.05),and it was significantly lower in poorly and moderately differentiated RCC tissues  than that in well differentiated group(P<0.05).In addition,in hMOF lower expression group,the risk of lymphonode and distant metastasis was increased.Conclusion With the increasing of Fuhrman grade,the expression of hMOF protein in kidney carcinoma  tissue is decreased significantly.The hMOF protein may be associated with the occurrence and development of kidney carcinoma,and malignant degree.

Key words: hMOF;renal cell carcinoma;immunohistochemisry；quantive , real time-polymerase chain reaction