Abstract:

Abstract:Objective To detect the expression of Twist1 protein in gastric carcinoma tissues,and to explore the clinicopathological significance of Twist1 protein in the carcinogenesis and progression of gastric carcinoma.Methods The expressions of Twist1 protein were detected by immunohistochemistry in 199 cases of gastric carcinomas,25 cases of high-grade intraepithelial neoplasia,24 cases of low-grade intraepithelial neoplasia,46 cases of intestinal metaplasia,17 cases of chronic atrophic gastritis and 23 cases of normal gastric mucosa.The relationships between the expression of Twist1 protein and clinicopathological characteristics of gastric carcinoma were analyzed.Results The expression of Twist1 protein in gastric carcinoma tissue was higher than those in intraepithelial neoplasia,chronic atrophic gastritis and normal gastric mucosa tissues,and there were  significant differences（P<0.001）.Meanwhile,the expression of Twist1 protein in tumor size ≥4 cm group was higher than that in tumor size <4 cm group（P<0.01），and it was higher in the patients with  lymph node metastasis than that in patients without metastasis（P<0.01）,and it was higher in the died patients  than that in lived patients （P<0.01）.However,the 　expression of Twist1 protein was not correlated with the type of gastric carcinoma,patient’s age,tumor location,T stage,tumor differentiation，clinical stage and  histological type of gastric carcinoma.Conclusion The expression of Twist1 in gastric carcinoma tissue is high,and it is related to tumor size and metastasis and prognosis,which may be an important factor in occurence of gastric carcinoma.

Key words: gastric neoplasms;Twist1;immunohistochemistry