Abstract:

Abstract:Objective To observe the changes  of the  levels of serum interleukin-10（IL-10）,plasma endothelin(ET-1) and immunoglobulin E (IgE) in the children with or without  atopic asthma and to discuss their  clinical significances.Methods 60  asthma children conducted the Allergen skin prick tests and serum IgE measurement to determine atopic status at the same time,and they were divided into atopic asthma group(n=32) and non-atopic asthma group(n=28) according to the results. 30  normal healthy children were selected as control group.The expressions of ET-1 and IgE in the asthma children during attack period and remission phase and the children in control group  were detected by radioimmunoassay and the  levels of serum IL-10 were detected by the double antibody sandwich ELISA.Results The  level of serum IL-10 in the asthma children in the acute attack period was lower than those in control group(P<0.01), and the level of ET-1 was higher obviously than that in control group(P<0.05);the levels of IgE in the asthma children in the acute attack period and remission phase in asthma groups were obviously higher than that in control group(P<0.01). The levels of ET-1 and IgE of the atopic asthma children in acute attack period were higher than those of the atopic asthma children in remission phase,and the level of IL-10 was lower(P<0.01).The levels of serum  IL-10 in atopic group of the acute attack period was lower than that in non-atopic group(P<0.05),and the levels of the ET-1 and IgE of the patients in  the acute attack period and remission phase in atopic group were higher than those in non-atopic group(P<0.05).The relationships between IL-10 level  and ET-1 and IgE showed  obviously negative correlations(r=－0.592，r=－0.894，P<0.05),and the relationship between ET-1 and IgE showed  obviously positive correlation(r=0.623，P<0.05).Conclusion The  levels of serum IL-10 and ET-1 perhaps take  part in the pathologic and physiological process of the children’s asthma and the changes of  the  levels of IL-10,ET-1 and IgE in the children with  atopic asthma  are more obvious.

Key words: bronchial asthma；interleukin-10； endothelin-1；immunoglobulin E