Abstract:

Abstract:Objective To investigate the relationships between transforming growth factor beta 1(TGF-β1),  polymorphism of human papilloma virus( HPV16) E6 gene and cervical carcinoma, and to clarify the possible mechanism of TGF-β1 in cervical carcinoma. Methods Fresh cervical tissues of hospitalized surgical patients between May 2007 to January 2009 were collected and divided into three groups, 17 cases of  cervical intraepithelial neoplasia Ⅰ(CIN Ⅰ);17 cases of CIN Ⅱ-Ⅲ;39 cases of cervical squamous cell carcinoma. The immunohistochemical method was used to observe the TGF-β1 protein expression in cervical tissues,and the direct PCR method was performed to test the genotype of HPV16 E6, finally the relationships between them and the occurrence and development of cervical carcinoma were analyzed. Results  The positive rates of TGF-β1 in CIN,CINⅡ-Ⅲ and  cervical squamous cell carcinoma group were 11.8% ,35.3% and 64.3%, respectively, and there were significant differences between various groups (P<0.01 ).The expression of TGF-β1 in cervical carcinoma  patients of stage ⅡB to ⅣA (88.9%) were significantly higher than that of stage ⅠB and ⅡA（76.7%）(P<0.01). The detection rates of HPV16　E6 in CINⅠ, CINⅡ-Ⅲ and cervical squamous cell carcioma group were 11.8%, 41.2% and 82.1%,there were significant differences between various groups(P<0.01). The positive rate of HPV16 E6 gene was positively related with the development of cervical carcinoma （r=0.592 ，P=0.001). By DNA sequencing, DNAman program was used to analyze the data, totally 14 sequences were the same as the standard strain，and the most common mutant site was D25E（42.5%). The TGF-β1 positive rates in HPV16 E6 non-mutant patients and in HPV16E6 D25E patients were 50.0% and 92.9%, respectively (P<0.05).Conclusion The occurrence and development of cervical carcinoma has something to do with TGF-β1 expression, and the HPV16　E6 polymorphism may be associated with the TGF-β1 which promote to growth and invasion of cancer.

Key words: uterine cervical neoplasms;transforming growth factor;human papilloma virus;E6 gene;gene polymorphism;polymerase chain reaction