Abstract:

Objective To investigate the association between the single polymorphisms(SNPs) of MMP-9 gene C1562T,R279Q and coronary heart disease (CHD). Methods 258 patients diagnosed with CHD and 183 healthy contols were selected. The serum levels of MMP-9 were determinated using enzyme-linked immunosorbent assay (ELISA). PCR-RFLP analysis was applied to detect the C1562T and R279Q SNPs of MMP-9 gene. Statistic
al soft ware SPSS 17.0 was used to analyze the frequency distribution of alleles and genotypes. Results The serum MMP-9 level  in CHD group was significantly higher than that in  control group（P<0.05）. The C1562T genotype frequencies of CT+TT genotypes and T allele were 24.4% and 11.8%  in CHD group and 13.8% and 7.3% in control group, respectively, and there were significant differences  between  two groups (P<0.05). The R279Q genotype frequencies of AG+GG genotypes and G allele were 60.2% and 41.7%  in CHD group and 68.0% and 41.5%  in control group, respectively, and there were no  significant differences between  two groups(
P>0.05). Conclusion The  MMP-9 level in patients with CHD  is increased and the polymorphisms in MMP-9 gene C1562T associates with the susceptibility to the CHD,but there is  no association between R279Q polymorphism and CHD.

Key words: coronary heart disease;marix metalloproteinase-9;gene polymorphism