Abstract:

Abstract:Objective
To build oxygen glucose deprivation/reperfusion(OGD/R) model in vitro to mimic ischemia and reperfusion inducing apoptosis of SH-SY5Y cells,to detect differential proteins by employing proteomics technique and to reveal the potential   mechanisms of neuronal apoptosis.Methods  SH-SY5Y cells were employed and divided into control group and experimental group in which the cells treated with OGD10h/R24h to set up apoptosis model.After the proteins were extracted from SH-SY5Y cells in control and OGD10h/R24h groups,2D-DIGE techniques was used to detect differential proteins.Results  By DeCyder software mean (1 800±156) protein spots were  displayed on 2D-DIGE image.In OGD10h/R24h group,30 protein spots were detected to  differentially express compared with control group,of which 22 spots were up-regulated and 8 spots were down-regulated.Compared with control groups at the same time, the differences were significant (P<0.05 or P<0.01).Conclusion  By employing 2D-DIGE proteomics techniques to analyse OGD/R model,the protein spots are detected to differentially express.

Key words: oxygen glucose deprivation/reperfusion, proteomics, 2-dimentional difference gel electrophoresis