Abstract:

Abstract:Objective To evaluate the inhibitory effect of Flavones of Buckwheat Compound(FBC )on the histomorphologic changes of the kidney tissue in type 2 diabeticrats,and to explore the mechanism of FBC on diabetic nephropathy(DN).Methods The type 2 diabetic male SD rat model was induced by feeding high-fat diet and intraperitoneal injection with streptozotocin (STZ).The rats were randomly divided into five groups: model group(distilled water,10 mL/kg/d),positive medicine group(XKW,0.83 g/kg/d),low and high doses FBC groups(FBC,0.6 and 1.2 g/kg/d),and normal control group(distilled water, 10  mL/kg/d).The rats in each group  were gavaged daily for eight weeks.At the end of the experiment,the level of fasting blood glucose(FBG) was detected and the index of kidney was observed.HE and Masson staining were used to observe the morphological changes of kidney tissue under optical microscope.The changes of the kidney tissue ultrastucture were observed using   electron microscope.Results  Compared with model group,the level of FBG and kidney index in FBC groups were significantly decreased（P<0.01).However there were no obvious differences in these indexes between control group and FBC groups.And FBC could inhibit the increasing of mesangial matrix and the thickening of basement membrane in kidney of diabetic rats,and relieve glomerular sclerosis and renal tubular epithelial cell vacuolation.Conclusion FBC could inhibit the increasing of mesangial matrix and basement membrane thickening of diabetic rats.The mechanism is related to inhibiting mesangial cell proliferation and glomerular basement membrane formation and improving the increasing of diabetic glomerular volume and hardening.

Key words: flavonoes of buckwheat compound;diabetic nephropathy;kidney