Abstract:

Objective To detect the expressions of MMP-14 and TIMP-2 in colorectal carcinoma tissues, and investigate the relationship between MMP-14, TIMP-2 and clinicopathological features.Methods  60 paraffin-embedded cancer specimens   from operated patients with colorectal carcinoma   were selected, and 20 normal mucous memb
ranes of colon and rectum were selected as control.The expressions of MMP-14 and TIMP-2 were detected by S-P immunohistochemistry.The relationship between the expressions of MMP-14, TIMP-2 and clinicopathological parameters were analyzed.Results  The expression rates of MMP-14 and TIMP-2 were 86.7% and 80.0%, respectively, in colorectal carcinoma tissues and were significantly higher than those in control group (P<0.01).The expression of MMP-14 was closely correlated with the depth of tumor invasion, lymph node metastasis and Dukes stage.The expression rates of MMP-14 in serosa group, lymph node metastasis group and Dukes C＋D stage were 89.5%, 94.1%, and 94.3%, respectively, and were significantly higher than those
 in non-serosa group, no lymph node metastasis group and Dukes A＋B stage (68.2%, 73.1%, and 76.0%)（P<0.05).The expression of TIMP-2 was closely correlated with the depth of tumor invasion, lymph node metastasis and Dukes stage.The expression rates of TIMP-2 in serosa group, lymph node metastasis group and Dukes C＋D stage were 55.3%, 64.7%, and 62.9%, respectively, and were significantly lower than
those in non-serosa group, no lymph node metastasis group and Dukes A＋B stage (81.8%, 88.5%, and 92.0%)(P<0.05).There was negative correlation between the expressions of MMP-14 and TIMP-2 in colorectal carcinoma tissues(rs＝－1.0, P<0.05). Conclusion  The high expression of MMP-14 in colorectal carcinoma tissues may contribute to tumor invasion and metastasis and  TIMP-2 may play an inhibitory effect  in the development of colorectal carcinoma. The imbanlance of MMP-14 and TIMP- 2 may be one of the mechanisms of tumor invasion and metastasis.
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Key words: matrix metalloproteinases-14, tissue inhibitors of metalloproteinase-2, colorectal neoplasms, invasion and metastasis