Abstract:

Objective To observe the promoting effect of inhibiting chloride channel-3(ClC-3) gene expression on the apoptosis of human ovarian cancer SKOV3/DDP cells induced by cisplatin (DDP),and to provide the experimental basis for   treatment of ovarian cancer.Methods The recombinant plamid pSH1-siRNA-ClC-3 was transfected into SKOV3/DDP cells.The ClC-3 expression changes in SKOV3/DDP cells transfected with pSH1-SiRNA-ClC-3 was detected by Western blotting.The vitality of SKOV3/DDP cells was measured by MTT assay.The expression levels of apoptosis-related proteins cytochrome C (Cyt-C) and Cleaved Caspase-3 were determined byWestern blotting. Results  Compared with SKOV3 cells,the ClC-3 expression levels  in SKOV3/DDP cells was increased (P<0.05).Transfection of pSH1-SiRNA-ClC-3 suppressed the expression of ClC-3 efficiently in SKOV3/DDP cells (P<0.05).The proliferation rate of SKOV3/DDP cells was  decreased (P<0.05),and the cyt-C and Cleaved Caspase-3 expression were increased  after treated with ClC-3-siRNA combined with  DDP(P<0.05).Conclusion pSH1-SiRNA-ClC-3 could suppress the expression of ClC-3 efficiently and enhance the apoptosis induced by DDP in SKOV3/DDP cells.

Key words:  ovarian tumor, chloride channel-3;cisplatin;apoptosis