Abstract:

Abstract:Objective
To observe the inhibitory effect of andrographolide (Andro) on cell proliferation of  glioblastoma U87 cells,and to explore its regulatory mechanism.Methods The U87 cells at logarithmic growth phase were collected and divided into dimethy1 sulfoxide(DMSO) control group,4H-Andro control group and Andro group.The U87 cells were treated with DMSO,4H-Andro,and Andro with  different concentrations (0,5,10,15,20,25 μmol•L^-1).The cell viability was detected by MTT assay,and the mean inhibitory concentraion (IC50) was caculated, and Western blotting method was used to detect the expression intensity  of NF-κB protein  in U87 cells.Results The U87 cells were treated with  different concentrations of Andro,and the  IC50 of Andro was 8 μmol•L^-1.Compared with DMSO and 4H-Andro control groups,24 h after treatment of 8 μmol•L^-1 Andro,the proliferation activity of U87 cells was inhibited (P<0.01) and the inhibitory rate of proliferation was 50%;and the cell proliferation was obviously decreased after treated with Andro for 96 h (P<0.01) in U87 cells,and the inhibitory rate of proliferation was 65%.The expression intensity of NF-κB(pp65) protein was  down-regulated by 8 μmol•L^-1 Andro for 48 h compared with DMSO control  group　and 4H-Andro control group(P<0.01).Conclusion Andro, as an anti-inflammatory drug in clinic,plays a new function of suppressing glioblastoma cell proliferation  through inhibiting the expression intensity  of NF-κB protein.
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Key words: androgapholide, glioblastoma cell, cell proliferation androgapholide, glioblastoma cell, cell proliferation