Abstract:

Objective To investigate the changes of peripheral blood CD4+CD25+
 regulatory T cells in patients with primary pancreatic cancer before and after
treatment of high intensity focused ultrasound(HIFU),and to elucidate the effects of HIFU treatment on immune fun
ction of the patients with primary pancreatic cancer. Methods
Flow cytometry was used to detect the percentage 
of CD4+CD25+ regulatory T cells in peripheral blood of the patients
in pancreatic carcinoma group(n=36) before and 4 weeks after HIFU treatment and healthy persons in control group(n=28).The KPS scores,the levels of CEA
 and CA-199 of the patients with primary pancreatic cancer before and after treatment were compared.
Results The percentage of CD4+CD25+ regulatory T cells of the patients  before HIFU treatment was significantly higher than that in control group,the difference was statistically significant (P<0.01).The percentage of CD4+CD25+ regulatory T cells of the patients in pancreatic cancer group 4 weeks after HIFU treatment was lower than  before treatment,the difference was statistically significant (P<0.01).The KPS score of 36 patients with primary pancreatic cancer after HIFU treatment was significantly increased compared with before treatment,the difference was statistically significant ( P<0.05 ).The
 levels  of CEA and CA-199 of the patients after HIFU treatment was significantly decreased compared with before treatment,the difference was statistically significant (P<0.05).Conclusion HIFU treatment can improve the cell immune function of the patients with pancreatic cancer.
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Key words: high intensity focused ultrasound, pancreatic neoplasms, CD4+CD25+ regulatory T cells