Abstract:

Objective
 To establish an high performance liquid chromatography （HPLC） method for the determination of concentrations of  five active ginsenosides （Rg1,Re,Rb1,Rb2,and Rd）in Life-Source Ginsenosides Complex,and to provide  basis for the quality control of industrial production.Methods HPLC method was used to determine the concentrations of five ginsenosides in Life-Source Ginsenosides Complex.The developed conditions of HPLC were as follows:   Alltima C18 (250.0 mm × 4.6 mm,5.0  μm） column;A gradient elution was applied by mixing mobile phase A and B,which consisted of acetonitrile and  0.1% phosphoric acid,respectively;the flow rate was 1.0 mL?min-1，and the detector wave length was 203 nm and the column temperature was 40℃.Results The calibration curve showed good linearity,and the values of correlation coefficient were higher than 0.999 1 for all the analytes,and the average recovery rates were ranged from 99.5% to 102.6%.The relative standard deviation (RSD) detected by  accuracy,stability and repetability tests of five ginsenosides were 0.35%-0.76%,0.74%-1.44%,and 0.78%-1.47%,respectively.Conclusion  The detection of ginsenosides in Life-Soure Ginsenosides Complex with HPLC method is accurate,easy and reproducible,which can be applied to quality control of the Life-Source Ginsenosides Complex.

Key words: Life-Source Ginsenosides Complex, ginsenoside, chromatography,high performance liquid