Abstract:

Abstract:Objective
To investigate the influence of the eye acupuncture therapy in the expressions of brain derived neurophic factor(BDNF) and tyrosine kinase receptor B(TrkB)  in penumbra brain tissue of ischemic area in the rats with acute cerebral ischemia reperfusion injury, and to explore the related mechanism of protective effect on brain of eye acupunture.Methods 62 SD rats were randomly divided into blank control group (n=11),sham operation group (n=11) and model copy  group (n=40). The rats in copy model group were used to establish models with suture method,and the  successful model    rats (n=32)  were randomly divided into model group（n=16）and eye acupuncture group(n=16).The rats in eye acupuncture group were treated with eye acupuncture intervention;the acupoints were chosen according to human acupoint selection method,the liver,the upper-jiao,the down-jiao district,and renal were selected for acupuncture intervention.After reperfusion for 2 h, the acupuncture was performed once every 8 h,lasted for  10 times;30 min after the last intervention,the rats were sacrificed;the regional ischemia penumbra around the parts of the brain tissue was obtained and the expressions of BDNF and TrkB mRNA and protein  in brain tissue of the rats were detected with RT-PCR and Western blotting method.Results Compared with  blank control group,the expression levels of BDNF and TrkB mRNA and protein  in brain tissue of the rats in sham operation group had no statistical significance（P>0.05）；compared with  sham operation group,the expression levels of BDNF and TrkB mRNA and protein  in brain tissue of the rats in model group were significantly up-regulated(P<0.01);compared with  model group,the expression levels of BDNF and TrkB mRNA and protein  in brain tissue of the rats in eye acupuncture group were significantly up-regulated（P<0.05 or P<0.01）.Conclusion The expressions of BDNF and TrkB are increased after cerebral ischemia and reperfusion injury.Eye acupuncture can protect the brain of MCAO/R rats,which may be related to the up-regulation of the expressions of BDNF and TrkB in the corresponding penumbra.

Key words: eye acupuncture, cerebral ischemia-reperfusion, brain-derived neurotrophic factor, tyrosine kinase receptor B