Abstract:

Abstract:Objective To investigate the effects of Yindan Xinnaotong Capsule on the osteoponin（OPN）expression in myocardium tissue of the rats after acute myocardial infarction(AMI) and to clarify the mechanism of Yindan Xinnaotong Capsule in alienating rat AMI.Methods 90 Wistar rats were used to establish AMI models by ligating of the left anterior descending coronary artery.The AMI model rats  were  randomly divided into　AMI model group,high dose of Yindan (16 g/kg/d) group,low dose of Yindan(0.08 g/kg/d) group,positive drug control captopril(5 mg/kg/d) group(n=12)；at the same time sham operation group(n=10) was set up,the rats in sham operation group was treated with wearing without ligation.All the rats were administrated for 4 weeks,then the myocardium tissue samples were obtained.The histological changes of myocardium tissue were observed by HE and Masson staining;the DNA fragments of apoptotic cells were detected by TUNEL staining and the apoptotic index(AI)was calculated.The expression of OPN mRNA in non-infarction area was measured by RT-PCR.Results Compared with sham operation group,the AI  of the rats in model group was significantly increased(P<0.05)； compared with model group,the AI of the rats in Yindan groups and captopril group were markedly decreased(P<0.01);compared with captopril group,the AI of the rats in high dose of Yindan group was significantly decreased(P<0.05).Compared with sham operation group,the expression level of OPN mRNA in non-infarction area of the rats in model group was significantly increased(P<0.01);compared with model group,the expression levels of OPN mRNA in non-infarction area of the rats in Yindan groups and captopril group were also decreased significantly(P<0.01);compared with captopril group,the expression level of OPN mRNA non-infarction area of the rats in high dose of Yindan group was significantly decreased(P<0.05).Conclusion Yindan Xinnaotong Capsule could obviously alleviate the apoptosis of myocardium tissue of the rats after acute myocardial infarction and decrease the expression of OPN mRNA in non-infarction area of left ventricle,which indicates that Yindan Xinnaotong Capsule may protect myocardium tissue through decreasing the OPN mRNA  expression.

Key words: Yindan Xinnaotong capsule, acute myocardial infarction, apoptosis, osteopontin