Abstract:

Objective To establish and evaluate the method to collect the  rat gingival crevicular fluid （GCF） by using absorbent paper points,and to lay foundati
on for analysis on GCF.Methods 20 healthy male rats were selected and randomly divided into GCF group and saliv
a group.The GCF of  the right upper molar gingival trough  of the rats in GCF group and the saliva of the rats in saliva group were collected by using 15# ab
sorbent paper points.The SDS-PAGE analysis  and abundance detection were applied to  analyze the protein bands of the sa
mples in two groups.Results The SDS-PAGE analysis identified the proteins at 77 000,66 000,55 000,51 000,and 28 000,especially 66 000 in GCF group.While saliva group had lower brightness protein bands at 66 000,60 000,and 48 000.The data of protein abundance of 66 000 between two groups had statistically significant difference (P<0.05). Conclusion The number and types of the protein bands are different between GCF Group and saliva group,so using 15# absorbent paper points can collect  the rat GCF successfully.

Key words: gingival crevicular fluid, absorbent paper points, protein analysis, rats,Wistar